Interactions between Rotavirus
and Suwannee River
Organic Matter: Aggregation, Deposition, and Adhesion Force Measurement
Leonardo Gutierrez
Thanh H. Nguyen
10.1021/es301336u.s001
https://acs.figshare.com/articles/journal_contribution/Interactions_between_Rotavirus_and_Suwannee_River_Organic_Matter_Aggregation_Deposition_and_Adhesion_Force_Measurement/2494435
Interactions between rotavirus and Suwannee River natural
organic
matter (NOM) were studied by time-resolved dynamic light scattering,
quartz crystal microbalance, and atomic force microscopy. In NOM-containing
NaCl solutions of up to 600 mM, rotavirus suspension remained stable
for over 4 h. Atomic force microscopy (AFM) measurement for interaction
force decay length at different ionic strengths showed that nonelectrostatic
repulsive forces were mainly responsible for eliminating aggregation
in NaCl solutions. Aggregation rates of rotavirus in solutions containing
20 mg C/L increased with divalent cation concentration until reaching
a critical coagulation concentration of 30 mM CaCl<sub>2</sub> or
70 mM MgCl<sub>2</sub>. Deposition kinetics of rotavirus on NOM-coated
silica surface was studied using quartz crystal microbalance. Experimental
attachment efficiencies for rotavirus adsorption to NOM-coated surface
in MgCl<sub>2</sub> solution were lower than in CaCl<sub>2</sub> solution
at a given divalent cation concentration. Stronger adhesion force
was measured for virus–virus and virus–NOM interactions
in CaCl<sub>2</sub> solution compared to those in MgCl<sub>2</sub> or NaCl solutions at the same ionic strength. This study suggested
that divalent cation complexation with carboxylate groups in NOM and
on virus surface was an important mechanism in the deposition and
aggregation kinetics of rotavirus.
2012-08-21 00:00:00
CaCl 2 solution
NaCl solutions
divalent cation complexation
Suwannee River Organic Matter
rotaviru
AFM
70 mM MgCl 2. Deposition kinetics
NOM
MgCl 2 solution
divalent cation concentration
interaction force decay length
Experimental attachment efficiencies
quartz crystal microbalance
Atomic force microscopy
surface
Adhesion Force MeasurementInteractions
30 mM CaCl 2
virus