Thiazoline Peptides and a Tris-Phenethyl Urea from <i>Didemnum molle</i> with Anti-HIV Activity Zhenyu Lu Mary Kay Harper Christopher D. Pond Louis R. Barrows Chris M. Ireland Ryan M. Van Wagoner 10.1021/np300270p.s001 https://acs.figshare.com/articles/journal_contribution/Thiazoline_Peptides_and_a_Tris_Phenethyl_Urea_from_i_Didemnum_molle_i_with_Anti_HIV_Activity/2493328 As part of our screening for anti-HIV agents from marine invertebrates, the MeOH extract of <i>Didemnum molle</i> was tested and showed moderate <i>in vitro</i> anti-HIV activity. Bioassay-guided fractionation of a large-scale extract allowed the identification of two new cyclopeptides, mollamides E and F (<b>1</b> and <b>2</b>), and one new tris-phenethyl urea, molleurea A (<b>3</b>). The absolute configurations were established using the advanced Marfey’s method. The three compounds were evaluated for anti-HIV activity in both an HIV integrase inhibition assay and a cytoprotective cell-based assay. Compound <b>2</b> was active in both assays with IC<sub>50</sub> values of 39 and 78 μM, respectively. Compound <b>3</b> was active only in the cytoprotective cell-based assay, with an IC<sub>50</sub> value of 60 μM. 2012-08-24 00:00:00 78 μ M IC 50 value Compound cytoprotective 60 μ M Didemnum molle HIV integrase inhibition assay IC 50 values