%0 Journal Article %A Li, Ting %A Ni, Siyang %A Jia, Changhong %A Wang, Hongyuan %A Sun, Guizhi %A Wu, Linzhuan %A Gan, Maoluo %A Shan, Guangzhi %A He, Weiqing %A Lin, Ling %A Zhou, Hongxia %A Wang, Yiguang %D 2012 %T Identification of 4,5-Dihydro-4-hydroxygeldanamycins As Shunt Products of Geldanamycin Biosynthesis %U https://acs.figshare.com/articles/journal_contribution/Identification_of_4_5_Dihydro_4_hydroxygeldanamycins_As_Shunt_Products_of_Geldanamycin_Biosynthesis/2493313 %R 10.1021/np3001738.s001 %2 https://acs.figshare.com/ndownloader/files/4136176 %K GDM biosynthesis %K Streptomyces hygroscopicus 17997. Compounds 1 %K cytochrome P 450 oxidase %X Two new geldanamycin (GDM) analogues, (4S)-4,5-dihydro-4-hydroxygeldanamycin (1) and (4R)-4,5-dihydro-4-hydroxygeldanamycin (2), were identified from Streptomyces hygroscopicus 17997. Compounds 1 and 2 were not normal intermediates of GDM biosynthesis but shunt products of C-4,5 oxidation catalyzed by GdmP, a cytochrome P450 oxidase acting as a desaturase in GDM biosynthesis. Preliminary assays implied that, compared with GDM, 1 and 2 exhibited decreased cytotoxicity. %I ACS Publications