%0 Journal Article %A Zachariasova, Milena %A Vaclavikova, Marta %A Lacina, Ondrej %A Vaclavik, Lukas %A Hajslova, Jana %D 2016 %T Deoxynivalenol Oligoglycosides: New “Masked” Fusarium Toxins Occurring in Malt, Beer, and Breadstuff %U https://acs.figshare.com/articles/journal_contribution/Deoxynivalenol_Oligoglycosides_New_Masked_Fusarium_Toxins_Occurring_in_Malt_Beer_and_Breadstuff/2487547 %R 10.1021/jf302069z.s001 %2 https://acs.figshare.com/ndownloader/files/4130335 %K preconcentration %K Fusarium %K BreadstuffThe %K presence %K Masked %K oligoglycosylated deoxynivalenols %K New %K strategy %K parent toxin %K chromatography %K fate %K deoxynivalenol issue %K hexose units %K bread baking %K origination %K Occurring %K Special attention %K deoxynivalenol conjugates %K health concern %K Malt %K bioavailability %K mycotoxin %K diet %K orbitrap mass spectrometry %K Deoxynivalenol Oligoglycosides %K malt %K preparation %K complexity %K Toxin %X The co-occurrence of deoxynivalenol-3-glucoside with its parent toxin, deoxynivalenol, has been recently documented in many cereal-based foods, especially in those produced by enzyme-catalyzed processes. The presence of this masked mycotoxin in the human diet has become an issue of health concern, mainly because of its assumed bioavailability. A selective immunoaffinity-based preconcentration strategy, followed by ultrahigh-performance liquid chromatography coupled with high-resolution orbitrap mass spectrometry, revealed that, in addition to the most common deoxynivalenol-3-glucoside, also oligoglycosylated deoxynivalenols with up to four bound hexose units were present in cereal-based products. The structure, origination, and fate of these deoxynivalenol conjugates during malt/beer production and bread baking have been thoroughly investigated. Special attention has been paid to the changes of deoxynivalenol conjugates enabled by industrial glycosidase-based enzymatic preparations. To the authors' best knowledge, this is the first study documenting the complexity of masked deoxynivalenol issue. %I ACS Publications