Immobilizing Penicillin
G Acylase Using Silica-Supported Ionic Liquids: The Effects of Ionic
Liquid Loadings
Huacong Zhou
Liangrong Yang
Wei Li
Fuchun Wang
Wensong Li
Junmei Zhao
Xiangfeng Liang
Huizhou Liu
10.1021/ie301843v.s001
https://acs.figshare.com/articles/journal_contribution/Immobilizing_Penicillin_G_Acylase_Using_Silica_Supported_Ionic_Liquids_The_Effects_of_Ionic_Liquid_Loadings/2478910
The supported ionic liquids (SILs) were prepared via
immobilizing silane-functionalized ionic liquids (IL) onto the monodispersive
silica nanoparticles, and the composite material was then applied
in the immobilization of penicillin G acylase (PGA, EC 3.5.1.11),
an important industrial enzyme for the production of semisynthetic
antibiotics. This was a novel approach of immobilizing PGA proposed
in our previous report, in which we had studied the effects of IL
types on the performance of this novel support. Here, we focused on
the effects of IL loading on the properties of the SILs and their
performance in PGA immobilization. The effects of silica sizes and
routes of preparing SILs (the solvent route and the sol–gel
route) on IL loading were investigated. The results showed that (1)
the IL loading could be tuned efficiently by changing the silica sizes.
(2) As compared with the solvent route, the sol–gel route is
more simple, time-saving, and ecofriendly in operation. What's more,
it has a higher IL loading. Among the factors affecting the IL loading,
the reaction time was the most feasible factor to control to tune
the IL loading, as compared with the temperature and the dosage of
ILs. (3) With the increase of IL loading, the surface ζ-potential
of silicas changed from negative to positive, and the isoelectronic
point (IEP) increased accordingly, which caused the decrease of the
protein loading but the increase of apparent and specific activity.
A reusability experiment showed that the sol–gel 1 sample maintained
approximately 55% of the initial activity even after 10 consecutive
operation cycles under the experimental conditions. This activity
was 3.7 times higher than that of the sol–gel 2 sample.
2012-10-10 00:00:00
SIL
silica sizes
monodispersive silica nanoparticles
EC
Ionic Liquid LoadingsThe
sol
IEP
penicillin G acylase
Immobilizing Penicillin G Acylase
IL loading
PGA