On-Cell MAS NMR: Physiological
Clues from Living Cells
Giorgia Zandomeneghi
Karin Ilg
Markus Aebi
Beat H. Meier
10.1021/ja307467p.s001
https://acs.figshare.com/articles/journal_contribution/On_Cell_MAS_NMR_Physiological_Clues_from_Living_Cells/2475178
While structural information on biomolecules is mainly
obtained
from purified in vitro samples, NMR can also be applied in the context
of entire cells or organisms. The present study describes maturation
processes in living <i>Salmonella enterica</i> serovar Typhimurium,
a prevalent cause for human gastroenteritis. In our physiological
study, we follow the composition of the O-antigen on the outer bacterial
membrane with high-resolution MAS NMR spectroscopy. We detect and
characterize an evolution of the O-antigen composition, in particular
of the O-acetylation state of the O-antigen, a factor that can play
an important role in vaccine development.
2012-10-24 00:00:00
Living CellsWhile
vaccine development
Physiological Clues
MAS NMR spectroscopy
maturation processes
Salmonella enterica serovar Typhimurium