%0 Journal Article
%A Gong, Yi-Jun
%A Zhang, Xiao-Bing
%A Zhang, Cui-Cui
%A Luo, Ai-Li
%A Fu, Ting
%A Tan, Weihong
%A Shen, Guo-Li
%A Yu, Ru-Qin
%D 2012
%T Through Bond Energy Transfer:
A Convenient and Universal
Strategy toward Efficient Ratiometric Fluorescent Probe for Bioimaging
Applications
%U https://acs.figshare.com/articles/journal_contribution/Through_Bond_Energy_Transfer_A_Convenient_and_Universal_Strategy_toward_Efficient_Ratiometric_Fluorescent_Probe_for_Bioimaging_Applications/2459521
%R 10.1021/ac302762d.s001
%2 https://acs.figshare.com/ndownloader/files/4102201
%K river water samples
%K overlap
%K wavelength difference
%K Hg
%K model metal ion
%K acceptor
%K energy transfer efficiency
%K Bond Energy Transfer
%K emission
%K bioimaging applications
%K Such TBET strategy
%K CR
%K FRET
%K Bioimaging ApplicationsFluorescence resonance energy transfer
%K donor
%K ratiometric probes
%X Fluorescence resonance energy transfer (FRET) strategy
has been
widely applied in designing ratiometric probes for bioimaging applications.
Unfortunately, for FRET systems, sufficiently large spectral overlap
is necessary between the donor emission and the acceptor absorption,
which would limit the resolution of double-channel images. The through-bond
energy transfer (TBET) system does not need spectral overlap between
donor and acceptor and could afford large wavelength difference between
the two emissions with improved imaging resolution and higher energy
transfer efficiency than that of the classical FRET system. It seems
to be more favorable for designing ratiometric probes for bioimaging
applications. In this paper, we have designed and synthesized a coumarin–rhodamine
(CR) TBET system and demonstrated that TBET is a convenient strategy
to design an efficient ratiometric fluorescent bioimaging probe for
metal ions. Such TBET strategy is also universal, since no spectral
overlap between the donor and the acceptor is necessary, and many
more dye pairs than that of FRET could be chosen for probe design.
As a proof-of-concept, Hg2+ was chosen as a model metal
ion. By combining TBET strategy with dual-switch design, the proposed
sensing platform shows two well-separated emission peaks with a wavelength
difference of 110 nm, high energy transfer efficiency, and a large
signal-to-background ratio, which affords a high sensitivity for the
probe with a detection limit of 7 nM for Hg2+. Moreover,
by employing an Hg2+-promoted desulfurization reaction
as recognition unit, the probe also shows a high selectivity to Hg2+. All these unique features make it particularly favorable
for ratiometric Hg2+ sensing and bioimaging applications.
It has been preliminarily used for a ratiometric image of Hg2+ in living cells and practical detection of Hg2+ in river
water samples with satisfying results.
%I ACS Publications