10.1021/nn3038594.s001
Sissel Juul
Sissel
Juul
Christine J. F. Nielsen
Christine
J. F. Nielsen
Rodrigo Labouriau
Rodrigo
Labouriau
Amit Roy
Amit
Roy
Cinzia Tesauro
Cinzia
Tesauro
Pia W. Jensen
Pia W.
Jensen
Charlotte Harmsen
Charlotte
Harmsen
Emil L. Kristoffersen
Emil L.
Kristoffersen
Ya-Ling Chiu
Ya-Ling
Chiu
Rikke Frøhlich
Rikke
Frøhlich
Paola Fiorani
Paola
Fiorani
Janet Cox-Singh
Janet
Cox-Singh
David Tordrup
David
Tordrup
Jørn Koch
Jørn
Koch
Anne-Lise Bienvenu
Anne-Lise
Bienvenu
Alessandro Desideri
Alessandro
Desideri
Stephane Picot
Stephane
Picot
Eskild Petersen
Eskild
Petersen
Kam W. Leong
Kam W.
Leong
Yi-Ping Ho
Yi-Ping
Ho
Magnus Stougaard
Magnus
Stougaard
Birgitta R. Knudsen
Birgitta R.
Knudsen
Droplet Microfluidics Platform for Highly Sensitive and Quantitative Detection of Malaria-Causing <i>Plasmodium</i> Parasites Based on Enzyme Activity Measurement
American Chemical Society
2012
droplet microfluidics platform
food quality control
detection limit
Droplet Microfluidics Platform
noninvasive saliva samples
Plasmodium parasites
Enzyme Activity MeasurementWe
DNA
Plasmodium enzyme topoisomerase
unprocessed blood
setup
years malaria transmission
Quantitative Detection
future adaptation
2012-12-21 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Droplet_Microfluidics_Platform_for_Highly_Sensitive_and_Quantitative_Detection_of_Malaria_Causing_i_Plasmodium_i_Parasites_Based_on_Enzyme_Activity_Measurement/2458333
We present an attractive new system for the specific and sensitive detection of the malaria-causing <i>Plasmodium</i> parasites. The system relies on isothermal conversion of single DNA cleavage–ligation events catalyzed specifically by the <i>Plasmodium</i> enzyme topoisomerase I to micrometer-sized products detectable at the single-molecule level. Combined with a droplet microfluidics lab-on-a-chip platform, this design allowed for sensitive, specific, and quantitative detection of all human-malaria-causing <i>Plasmodium</i> species in single drops of unprocessed blood with a detection limit of less than one parasite/μL. Moreover, the setup allowed for detection of <i>Plasmodium</i> parasites in noninvasive saliva samples from infected patients. During recent years malaria transmission has declined worldwide, and with this the number of patients with low-parasite density has increased. Consequently, the need for accurate detection of even a few parasites is becoming increasingly important for the continued combat against the disease. We believe that the presented droplet microfluidics platform, which has a high potential for adaptation to point-of-care setups suitable for low-resource settings, may contribute significantly to meet this demand. Moreover, potential future adaptation of the presented setup for the detection of other microorganisms may form the basis for the development of a more generic platform for diagnosis, fresh water or food quality control, or other purposes within applied or basic science.