10.1021/es4006509.s001 Jerry E. Ongerth Jerry E. Ongerth LT2 <i>Cryptosporidium</i> Data: What Do They Tell Us about <i>Cryptosporidium</i> in Surface Water in the United States? American Chemical Society 2013 EPA location United States Federal regulation water quality parameters surface water source sample LT 2 Cryptosporidium Data surface water U.S data 2013-05-07 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/LT2_i_Cryptosporidium_i_Data_What_Do_They_Tell_Us_about_i_Cryptosporidium_i_in_Surface_Water_in_the_United_States_/2417812 Beginning in 2006 a United States Federal regulation required public water suppliers using surface water serving more than 10,000 population to analyze for <i>Cryptosporidium</i> in at least 24 consecutive monthly samples from each surface water source. In July 2012, the U.S. EPA released the resulting data consisting of ca. 45,000 records. No <i>Cryptosporidium</i> were found in 93% of samples and no <i>Cryptosporidium</i> were found in any samples analyzed from over half of 1670 locations sampled. Nevertheless, at 250 locations representing every region of the U.S., <i>Cryptosporidium</i> were found in sufficient numbers of samples to provide a picture of their occurrence nationwide. Data from about 100 sites reporting the highest numbers were examined in detail. Although analysis of matrix spikes was required for quality control, the results do not permit estimating organism concentrations. The data reported at each of the individual sample locations were analyzed in the form of cumulative probability distributions to describe key risk-related features of median level and variability. Taken as a whole, the data describe a spectrum of median <i>Cryptosporidium</i> occurrence in surface waters of the U.S. ranging from ca. 0.005 to ca. 0.5 oocysts/L. The variability at individual sites ranged from ca. 1 to 15 r.s.d. Based on the LT2 positive data, comparison to measurements of other water quality parameters, and independent means of estimating organism production from watersheds reported in the literature, the hypothesis is offered that <i>Cryptosporidium</i> may be found in surface water anywhere worldwide continuously and within the spectrum defined above.