10.1021/ac400479j.s001
Richard
H. Perry
Richard
H.
Perry
David I. Bellovin
David I.
Bellovin
Emelyn H. Shroff
Emelyn H.
Shroff
Ali I. Ismail
Ali I.
Ismail
Tahera Zabuawala
Tahera
Zabuawala
Dean W. Felsher
Dean W.
Felsher
Richard N. Zare
Richard N.
Zare
Characterization
of MYC-Induced Tumorigenesis by in
Situ Lipid Profiling
American Chemical Society
2013
tumorigenesi
Situ Lipid ProfilingWe
tissue
oncogene
strategy
onset
lipidomic
gene
200 μ m
approach
profile
ability
desorption electrospray ionization mass spectrometry imaging
HCC
carcinoma
MYC
Tumorigenesi
hepatocellular
Characterization
lipid molecules
tumor
model
2013-05-07 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Characterization_of_MYC_Induced_Tumorigenesis_by_in_Situ_Lipid_Profiling/2417404
We apply desorption electrospray
ionization mass spectrometry imaging
(DESI-MSI) to provide an in situ lipidomic profile of genetically
modified tissues from a conditional transgenic mouse model of MYC-induced
hepatocellular carcinoma (HCC). This unique, label-free approach of
combining DESI-MSI with the ability to turn specific genes on and
off has led to the discovery of highly specific lipid molecules associated
with MYC-induced tumor onset. We are able to distinguish normal from
MYC-induced malignant cells. Our approach provides a strategy to define
a precise molecular picture at a resolution of about 200 μm
that may be useful in identifying lipid molecules that define how
the MYC oncogene initiates and maintains tumorigenesis.