10.1021/ac400479j.s001 Richard H. Perry Richard H. Perry David I. Bellovin David I. Bellovin Emelyn H. Shroff Emelyn H. Shroff Ali I. Ismail Ali I. Ismail Tahera Zabuawala Tahera Zabuawala Dean W. Felsher Dean W. Felsher Richard N. Zare Richard N. Zare Characterization of MYC-Induced Tumorigenesis by in Situ Lipid Profiling American Chemical Society 2013 tumorigenesi Situ Lipid ProfilingWe tissue oncogene strategy onset lipidomic gene 200 μ m approach profile ability desorption electrospray ionization mass spectrometry imaging HCC carcinoma MYC Tumorigenesi hepatocellular Characterization lipid molecules tumor model 2013-05-07 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Characterization_of_MYC_Induced_Tumorigenesis_by_in_Situ_Lipid_Profiling/2417404 We apply desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to provide an in situ lipidomic profile of genetically modified tissues from a conditional transgenic mouse model of MYC-induced hepatocellular carcinoma (HCC). This unique, label-free approach of combining DESI-MSI with the ability to turn specific genes on and off has led to the discovery of highly specific lipid molecules associated with MYC-induced tumor onset. We are able to distinguish normal from MYC-induced malignant cells. Our approach provides a strategy to define a precise molecular picture at a resolution of about 200 μm that may be useful in identifying lipid molecules that define how the MYC oncogene initiates and maintains tumorigenesis.