Differential Regulation of Host Genes Including Hepatic
Fatty Acid Synthase in HBV-Transgenic Mice
Hongmin Zhang
Hong Li
Yixuan Yang
Sanglin Li
Hong Ren
Dazhi Zhang
Huaidong Hu
10.1021/pr400247f.s001
https://acs.figshare.com/articles/journal_contribution/Differential_Regulation_of_Host_Genes_Including_Hepatic_Fatty_Acid_Synthase_in_HBV_Transgenic_Mice/2408866
Hepatitis B virus (HBV) is the most common of the hepatitis viruses
that cause chronic liver infections in humans, and it is considered
to be a major global health problem. To gain a better understanding
of HBV pathogenesis, and identify novel putative targets for anti-HBV
therapy, this study was designed to elucidate the differential expression
of host proteins in liver tissue from HBV-transgenic mice. Liver samples
from two groups, (1) HBV-transgenic (Tg) mice, (2) corresponding background
normal mice, wild-type (WT) mice, were collected and subjected to
iTRAQ and mass spectrometry analysis. In total, 1950 unique proteins
were identified, and 68 proteins were found to be differentially expressed
in HBV-Tg mice as compared with that in WT mice. Several differentially
expressed proteins were further validated by real-time quantitative
RT-PCR, Western blot and immunohistochemical analysis. Furthermore,
the association of HBV replication with fatty acid synthase (FASN),
one of the highly expressed proteins in HBV-Tg mice, was verified.
Silencing of FASN expression in HepG2.2.15 cells suppressed viral
replication through the IFN signaling pathway, and some downstream
antiviral effectors. The implicated role of FASN in HBV replication
provides an opportunity to test existing compounds against FASN for
adjuvant therapy and/or treatment of HBV replication.
2013-06-07 00:00:00
WT
mass spectrometry analysis
IFN
HBV replication
FASN
HepG 2.2.15 cells
protein