Chen, Bo Jia, Yi Zhao, Jie Li, Hong Dong, Weiguang Li, Junbai Assembled Hemoglobin and Catalase Nanotubes for the Treatment of Oxidative Stress Hemoglobin-based nanotubes for the treatment of oxidative stress are successfully fabricated via covalent layer-by-layer assembly. Catalase, the most efficient enzyme for scavenging the hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), is embedded into the inner pores of the hemoglobin/dialdehyde heparin (Hb/DHP)<sub>5</sub> nanotubes via covalent bonding to protect the delicate enzyme from inactivation after the removal of the polycarbonate (PC) template. Dialdehyde heparin (DHP) as wall components is a cross-linker. The biocompatibility and capabilities of the nanotubes to protect the cells under oxidative stress are characterized with the Cell Counting Kit-8 (CCK-8). The scavenging capabilities of four kinds of autofluorescent nanotubes are tested through the reaction with H<sub>2</sub>O<sub>2</sub> by the measurement of the UV–vis absorbance at 240 nm. All the experimental results show that the assembled hemoglobin-based nanotubes possess H<sub>2</sub>O<sub>2</sub>-scavenging capacity as well as biocompatibility, indicating the potential application in the treatment of oxidative stress. CCK;covalent;capability;Catalase;PC;enzyme;H 2O;DHP;heparin;UV;nanotube;biocompatibility;oxidative stress;H 2O capacity 2013-09-26
    https://acs.figshare.com/articles/journal_contribution/Assembled_Hemoglobin_and_Catalase_Nanotubes_for_the_Treatment_of_Oxidative_Stress/2373013
10.1021/jp407164q.s001