Proteome Analysis of Cold Response in Spring and Winter
Wheat (<i>Triticum aestivum</i>) Crowns Reveals Similarities
in Stress Adaptation and Differences in Regulatory Processes between
the Growth Habits
Klára Kosová
Pavel Vítámvás
Sébastien Planchon
Jenny Renaut
Radomíra Vanková
Ilja Tom Prášil
10.1021/pr400600g.s001
https://acs.figshare.com/articles/dataset/Proteome_Analysis_of_Cold_Response_in_Spring_and_Winter_Wheat_i_Triticum_aestivum_i_Crowns_Reveals_Similarities_in_Stress_Adaptation_and_Differences_in_Regulatory_Processes_between_the_Growth_Habits/2361724
A proteomic
response to cold treatment (4 °C) has been studied
in crowns of a frost-tolerant winter wheat cultivar Samanta and a
frost-sensitive spring wheat cultivar Sandra after short-term (3 days)
and long-term (21 days) cold treatments. Densitometric analysis of
2-D differential in gel electrophoresis (2D-DIGE) gels has resulted
in the detection of 386 differentially abundant protein spots, which
reveal at least a two-fold change between experimental variants. Of
these, 58 representative protein spots have been selected for MALDI-TOF/TOF
identification, and 36 proteins have been identified. The identified
proteins with an increased relative abundance upon cold in both growth
habits include proteins involved in carbohydrate catabolism (glycolysis
enzymes), redox metabolism (thioredoxin-dependent peroxidase), chaperones,
as well as defense-related proteins (protein revealing similarity
to thaumatin). Proteins exhibiting a cold-induced increase in the
winter cultivar include proteins involved in regulation of stress
response and development (germin E, lectin VER2), while proteins showing
a cold-induced increase in the spring cultivar include proteins involved
in restoration of cell division and plant growth (eIF5A2, glycine-rich
RNA-binding protein, adenine phosphoribosyltransferase). These results
provide new insights into cold acclimation in spring and winter wheat
at the proteome level and enrich our previous work aimed at phytohormone
dynamics in the same plant material.
2013-11-01 00:00:00
Proteome Analysis
5A
adenine phosphoribosyltransferase
stress response
36 proteins
Cold Response
58 representative protein spots
winter wheat
germin E
protein spots
Growth HabitsA proteomic response
winter cultivar
spring cultivar
Densitometric analysis
cell division
plant growth
Stress Adaptation
VER
386 differentially
carbohydrate catabolism
proteome level
Regulatory Processes
plant material
Winter Wheat
Triticum aestivum
phytohormone dynamics
growth habits