Quantitative Proteomic and
Transcriptomic Analyses
of Molecular Mechanisms Associated with Low Silk Production in Silkworm <i>Bombyx mori</i>
Shao-hua Wang
Zheng-ying You
Lu-peng Ye
Jiaqian Che
Qiujie Qian
Yohei Nanjo
Setsuko Komatsu
Bo-xiong Zhong
10.1021/pr4008333.s010
https://acs.figshare.com/articles/dataset/Quantitative_Proteomic_and_Transcriptomic_Analyses_of_Molecular_Mechanisms_Associated_with_Low_Silk_Production_in_Silkworm_i_Bombyx_mori_i_/2325757
To
investigate the molecular mechanisms underlying the low fibroin
production of the ZB silkworm strain, we used both SDS-PAGE-based
and gel-free-based proteomic techniques and transcriptomic sequencing
technique. Combining the data from two different proteomic techniques
was preferable in the characterization of the differences between
the ZB silkworm strain and the original Lan10 silkworm strain. The
correlation analysis showed that the individual protein and transcript
were not corresponded well, however, the differentially changed proteins
and transcripts showed similar regulated direction in function at
the pathway level. In the ZB strain, numerous ribosomal proteins and
transcripts were down-regulated, along with the transcripts of translational
related elongation factors and genes of important components of fibroin.
The proteasome pathway was significantly enhanced in the ZB strain,
indicating that protein degradation began on the third day of fifth
instar when fibroin would have been produced in the Lan10 strain normally
and plentifully. From proteome and transcriptome levels of the ZB
strain, the energy-metabolism-related pathways, oxidative phosphorylation,
glycolysis/gluconeogenesis, and citrate cycle were enhanced, suggesting
that the energy metabolism was vigorous in the ZB strain, while the
silk production was low. This may due to the inefficient energy employment
in fibroin synthesis in the ZB strain. These results suggest that
the reason for the decreasing of the silk production might be related
to the decreased ability of fibroin synthesis, the degradation of
proteins, and the inefficiency of the energy exploiting.
2014-02-07 00:00:00
silk production
Molecular Mechanisms Associated
fibroin synthesis
Lan 10 strain
Low Silk Production
transcriptomic sequencing technique
Lan 10 silkworm strain
ZB strain
ZB silkworm strain
Silkworm Bombyx moriTo
protein