Identification of Virulence
Factors in <i>Leishmania infantum</i> Strains by a Proteomic
Approach
Simone da Fonseca
Pires
Luiz Carlos Fialho
Soraia
Oliveira Silva
Maria Norma Melo
Carolina Carvalho de Souza
Wagner Luiz Tafuri
Oscar Bruna Romero
Hélida Monteiro de Andrade
10.1021/pr400923g.s002
https://acs.figshare.com/articles/journal_contribution/Identification_of_Virulence_Factors_in_i_Leishmania_infantum_i_Strains_by_a_Proteomic_Approach/2309308
Knowledge
of <i>Leishmania</i> virulence is essential for understanding
how the contact between the pathogen and host cells can lead to pathogenesis.
Virulence in two <i>L. infantum</i> strains was characterized
using macrophages and hamsters. Next, we used difference gel electrophoresis
(DIGE) and mass spectrometry to identify the differentially expressed
proteins. A total of 63 spots were identified corresponding to 36
proteins; 20 were up-regulated, in which 16 had been previously associated
with <i>Leishmania</i> virulence. Considering our results
and what has been reported before, we suggest the hypothesis that <i>L. infatum</i> virulence could be a result of the increased
expression of KMP-11 and metallopeptidase, associated with an improved
parasite–host interacting efficiency and degradation of the
protective host proteins and peptides, respectively. Other factors
are tryparedoxin peroxidase and peroxidoxin, which protect the parasite
against the stress response, and 14-3-3 protein-like, which can prolong
infected host cell lifetime. Proteins as chaperones and endoribonuclease
L-PSP can increase parasite survival. Enolase is able to perform versatile
functions in the cell, acting as a chaperone or in the transcription
process, or as a plasminogen receptor or in cell migration events.
As expected in more invasive cells with high replication rates, energy
consumption and protein synthesis are higher, with up-regulation of
Rieske iron–sulfur protein precursor, EF-2, <i>S</i>-adenosylhomocysteine, and phosphomannomutase.
2014-04-04 00:00:00
Leishmania virulence
parasite
difference gel electrophoresis
EF
DIGE
KMP
Leishmania infantum Strains
cell migration events
protein
host cell lifetime