10.1021/pr400880s.s002
Mimi C. Yung
Mimi C.
Yung
Jincai Ma
Jincai
Ma
Michelle R. Salemi
Michelle R.
Salemi
Brett
S. Phinney
Brett
S.
Phinney
Grant R. Bowman
Grant R.
Bowman
Yongqin Jiao
Yongqin
Jiao
Shotgun Proteomic Analysis
Unveils Survival and Detoxification
Strategies by <i>Caulobacter crescentus</i> during Exposure
to Uranium, Chromium, and Cadmium
American Chemical Society
2014
bacterium Caulobacter crescentus
oxidative stress proteins
Heat shock
oxidative phosphorylation
cell cycle progression
promoter regions
Shotgun Proteomic Analysis Unveils Survival
phytase enzyme
bioremediation applications
acid metabolism
Detoxification Strategies
proteomic enrichment
cadmium sulfate
uranyl nitrate
Clustering analysis
Cr
Caulobacter crescentus
membrane responses
ABC transporter
efflux pumps
2014-04-04 00:00:00
Dataset
https://acs.figshare.com/articles/dataset/Shotgun_Proteomic_Analysis_Unveils_Survival_and_Detoxification_Strategies_by_i_Caulobacter_crescentus_i_during_Exposure_to_Uranium_Chromium_and_Cadmium/2309305
The
ubiquitous bacterium <i>Caulobacter crescentus</i> holds
promise to be used in bioremediation applications due to its
ability to mineralize U(VI) under aerobic conditions. Here, cell free
extracts of <i>C. crescentus</i> grown in the presence of
uranyl nitrate [U(VI)], potassium chromate [Cr(VI)], or cadmium sulfate
[Cd(II)] were used for label-free proteomic analysis. Proteins involved
in two-component signaling and amino acid metabolism were up-regulated
in response to all three metals, and proteins involved in aerobic
oxidative phosphorylation and chemotaxis were down-regulated under
these conditions. Clustering analysis of proteomic enrichment revealed
that the three metals also induce distinct patterns of up- or down-regulated
expression among different functional classes of proteins. Under U(VI)
exposure, a phytase enzyme and an ABC transporter were up-regulated.
Heat shock and outer membrane responses were found associated with
Cr(VI), while efflux pumps and oxidative stress proteins were up-regulated
with Cd(II). Experimental validations were performed on select proteins.
We found that a phytase plays a role in U(VI) and Cr(VI) resistance
and detoxification and that a Cd(II)-specific transporter confers
Cd(II) resistance. Interestingly, analysis of promoter regions in
genes associated with differentially expressed proteins suggests that
U(VI) exposure affects cell cycle progression.