%0 Journal Article %A Yung, Mimi C. %A Ma, Jincai %A Salemi, Michelle R. %A Phinney, Brett S. %A Bowman, Grant R. %A Jiao, Yongqin %D 2014 %T Shotgun Proteomic Analysis Unveils Survival and Detoxification Strategies by Caulobacter crescentus during Exposure to Uranium, Chromium, and Cadmium %U https://acs.figshare.com/articles/journal_contribution/Shotgun_Proteomic_Analysis_Unveils_Survival_and_Detoxification_Strategies_by_i_Caulobacter_crescentus_i_during_Exposure_to_Uranium_Chromium_and_Cadmium/2309302 %R 10.1021/pr400880s.s001 %2 https://acs.figshare.com/ndownloader/files/3946930 %K bacterium Caulobacter crescentus %K oxidative stress proteins %K Heat shock %K oxidative phosphorylation %K cell cycle progression %K promoter regions %K Shotgun Proteomic Analysis Unveils Survival %K phytase enzyme %K bioremediation applications %K acid metabolism %K Detoxification Strategies %K proteomic enrichment %K cadmium sulfate %K uranyl nitrate %K Clustering analysis %K Cr %K Caulobacter crescentus %K membrane responses %K ABC transporter %K efflux pumps %X The ubiquitous bacterium Caulobacter crescentus holds promise to be used in bioremediation applications due to its ability to mineralize U­(VI) under aerobic conditions. Here, cell free extracts of C. crescentus grown in the presence of uranyl nitrate [U­(VI)], potassium chromate [Cr­(VI)], or cadmium sulfate [Cd­(II)] were used for label-free proteomic analysis. Proteins involved in two-component signaling and amino acid metabolism were up-regulated in response to all three metals, and proteins involved in aerobic oxidative phosphorylation and chemotaxis were down-regulated under these conditions. Clustering analysis of proteomic enrichment revealed that the three metals also induce distinct patterns of up- or down-regulated expression among different functional classes of proteins. Under U­(VI) exposure, a phytase enzyme and an ABC transporter were up-regulated. Heat shock and outer membrane responses were found associated with Cr­(VI), while efflux pumps and oxidative stress proteins were up-regulated with Cd­(II). Experimental validations were performed on select proteins. We found that a phytase plays a role in U­(VI) and Cr­(VI) resistance and detoxification and that a Cd­(II)-specific transporter confers Cd­(II) resistance. Interestingly, analysis of promoter regions in genes associated with differentially expressed proteins suggests that U­(VI) exposure affects cell cycle progression. %I ACS Publications