Aarhus Sensor Green: A Fluorescent Probe for Singlet Oxygen Stephan K. Pedersen Jeppe Holmehave Frances H. Blaikie Anita Gollmer Thomas Breitenbach Henrik H. Jensen Peter R. Ogilby 10.1021/jo500219y.s001 https://acs.figshare.com/articles/journal_contribution/Aarhus_Sensor_Green_A_Fluorescent_Probe_for_Singlet_Oxygen/2309002 A tetrafluoro-substituted fluorescein derivative covalently linked to a 9,10-diphenyl anthracene moiety has been synthesized, and its photophysical properties have been characterized. This compound, denoted Aarhus Sensor Green (ASG), has distinct advantages for use as a fluorescent probe for singlet molecular oxygen, O<sub>2</sub>(a<sup>1</sup>Δ<sub>g</sub>). In the least, ASG overcomes several limitations inherent to the use of the related commercially available product called Singlet Oxygen Sensor Green (SOSG). The functional behavior of both ASG and SOSG derives from the fact that these weakly fluorescent compounds rapidly react with singlet oxygen via a <sub>π</sub>2 + <sub>π</sub>4 cycloaddition to irreversibly yield a highly fluorescent endoperoxide. The principal advantage of ASG over SOSG is that, at physiological pH values, both ASG and the ASG endoperoxide (ASG-EP) do not themselves photosensitize the production of singlet oxygen. As such, ASG better fits the requirement of being a benign probe. Although ASG readily enters a mammalian cell (i.e., HeLa) and responds to the presence of intracellular singlet oxygen, its behavior in this arguably complicated environment requires further investigation. 2014-04-04 00:00:00 SOSG intracellular singlet oxygen ASG singlet oxygen Aarhus Sensor Green Singlet Oxygen Sensor Green