Aggregation Behavior of Cationic Nanohydrogel Particles
in Human Blood Serum
Lutz Nuhn
Sabine Gietzen
Kristin Mohr
Karl Fischer
Kazuko Toh
Kanjiro Miyata
Yu Matsumoto
Kazunori Kataoka
Manfred Schmidt
Rudolf Zentel
10.1021/bm500199h.s001
https://acs.figshare.com/articles/journal_contribution/Aggregation_Behavior_of_Cationic_Nanohydrogel_Particles_in_Human_Blood_Serum/2306194
For
systemic siRNA delivery applications, well-defined drug carriers
are required that guarantee stability for both carrier and cargo.
Among various concepts progressing in market or final development,
cationic nanohydrogel particles may serve as novel transport media
especially designed for siRNA-in vivo experiments. In this work, the
interaction of nanohydrogel particles with proteins and serum components
was studied via dynamic light scattering in human blood serum as novel
screening method prior to applications in vivo. The formation of larger
aggregates mostly caused by charge interaction with albumin could
be suppressed by nanogel loading with siRNA affording a neutral zeta
potential for the complex. Preliminary in vivo studies confirmed the
results inside the light-scattering cuvette. Although both carrier
and cargo may have limited stability on their own under physiological
relevant conditions, they can form safe and stable complexes at a
charge neutralized ratio and thus making them applicable to systemic
siRNA delivery.
2014-04-14 00:00:00
nanogel loading
novel screening method
Aggregation Behavior
vivo studies
nanohydrogel particles
cargo
Human Blood SerumFor
charge interaction
siRNA delivery applications
drug carriers
novel transport media
siRNA delivery
Cationic Nanohydrogel Particles
serum components
blood serum
guarantee stability
cationic nanohydrogel particles