Homogeneous and Sensitive Detection of microRNA
with Ligase Chain Reaction and Lambda Exonuclease-Assisted Cationic
Conjugated Polymer Biosensing
Zheng Yuan
Yuanyuan Zhou
Shuxin Gao
Yongqiang Cheng
Zhengping Li
10.1021/am500883q.s001
https://acs.figshare.com/articles/journal_contribution/Homogeneous_and_Sensitive_Detection_of_microRNA_with_Ligase_Chain_Reaction_and_Lambda_Exonuclease_Assisted_Cationic_Conjugated_Polymer_Biosensing/2295151
A simple and homogeneous microRNA
assay is developed by integration of ligase chain reaction (LCR) and
lambda exonuclease-assisted cationic conjugated polymer (CCP) biosensing.
LCR is utilized for exponential amplification of microRNA, and lambda
exonuclease is introduced to degrade excess fluorescein-labeled probes
in LCR for eliminating background signal. After addition of CCP, efficient
fluorescence resonance energy transfer from CCP to fluorescein in
LCR products occurs. The method is sensitive enough to detect 0.1
fM target microRNA and specific to discriminate one-base difference
of microRNAs, which paves a new way for homogeneous microRNA detection
and molecular diagnosis.
2014-05-14 00:00:00
microRNA assay
LCR products
fluorescence resonance energy transfer
CCP
Sensitive Detection
background signal
microRNA detection
lambda exonuclease
0.1 fM target microRNA
Ligase Chain Reaction
ligase chain reaction