Homogeneous and Sensitive Detection of microRNA with Ligase Chain Reaction and Lambda Exonuclease-Assisted Cationic Conjugated Polymer Biosensing Zheng Yuan Yuanyuan Zhou Shuxin Gao Yongqiang Cheng Zhengping Li 10.1021/am500883q.s001 https://acs.figshare.com/articles/journal_contribution/Homogeneous_and_Sensitive_Detection_of_microRNA_with_Ligase_Chain_Reaction_and_Lambda_Exonuclease_Assisted_Cationic_Conjugated_Polymer_Biosensing/2295151 A simple and homogeneous microRNA assay is developed by integration of ligase chain reaction (LCR) and lambda exonuclease-assisted cationic conjugated polymer (CCP) biosensing. LCR is utilized for exponential amplification of microRNA, and lambda exonuclease is introduced to degrade excess fluorescein-labeled probes in LCR for eliminating background signal. After addition of CCP, efficient fluorescence resonance energy transfer from CCP to fluorescein in LCR products occurs. The method is sensitive enough to detect 0.1 fM target microRNA and specific to discriminate one-base difference of microRNAs, which paves a new way for homogeneous microRNA detection and molecular diagnosis. 2014-05-14 00:00:00 microRNA assay LCR products fluorescence resonance energy transfer CCP Sensitive Detection background signal microRNA detection lambda exonuclease 0.1 fM target microRNA Ligase Chain Reaction ligase chain reaction