Efficient Functionalization of Oligonucleotides by New Achiral Nonnucleosidic Monomers Maxim S. Kupryushkin Mikhail D. Nekrasov Dmitry A. Stetsenko Dmitrii V. Pyshnyi 10.1021/ol500668n.s002 https://acs.figshare.com/articles/journal_contribution/Efficient_Functionalization_of_Oligonucleotides_by_New_Achiral_Nonnucleosidic_Monomers/2285476 A novel synthetic strategy has been designed for preparation of achiral nonnucleosidic phosphoramidite monomers for automated solid-phase oligonucleotide synthesis. It is based on <i>O</i>-DMTr-protected 4-(2-hydroxyethyl)-morpholine-2,3-dione as the key compound and a family of building blocks obtained by its ring-opening by primary aliphatic amines. A series of nonnucleosidic phosphoramidites containing various side-chain functionalities was synthesized, and corresponding oligodeoxyribonucleotides incorporating modified units in single or multiple positions along the chain were prepared. 2014-06-06 00:00:00 building blocks series New Achiral Nonnucleosidic MonomersA novel hydroxyethyl aliphatic amines strategy Oligonucleotide synthesis functionalitie nonnucleosidic phosphoramidites oligonucleotide compound achiral nonnucleosidic phosphoramidite monomers Efficient Functionalization preparation oligodeoxyribonucleotide