<sup>1</sup>H NMR Metabolomics Reveals Contrasting
Response by Male and Female Mussels Exposed to Reduced Seawater pH,
Increased Temperature, and a Pathogen
Robert P. Ellis
John I. Spicer
Jonathan J. Byrne
Ulf Sommer
Mark R. Viant
Daniel A. White
Steve Widdicombe
10.1021/es501601w.s001
https://acs.figshare.com/articles/journal_contribution/_sup_1_sup_H_NMR_Metabolomics_Reveals_Contrasting_Response_by_Male_and_Female_Mussels_Exposed_to_Reduced_Seawater_pH_Increased_Temperature_and_a_Pathogen/2282122
Human
activities are fundamentally altering the chemistry of the
world’s oceans. Ocean acidification (OA) is occurring against
a background of warming and an increasing occurrence of disease outbreaks,
posing a significant threat to marine organisms, communities, and
ecosystems. In the current study, <sup>1</sup>H NMR spectroscopy was
used to investigate the response of the blue mussel, Mytilus edulis, to a 90-day exposure to reduced seawater
pH and increased temperature, followed by a subsequent pathogenic
challenge. Analysis of the metabolome revealed significant differences
between male and female organisms. Furthermore, males and females
are shown to respond differently to environmental stress. While males
were significantly affected by reduced seawater pH, increased temperature,
and a bacterial challenge, it was only a reduction in seawater pH
that impacted females. Despite impacting males and females differently,
stressors seem to act via a generalized stress response impacting
both energy metabolism and osmotic balance in both sexes. This study
therefore has important implications for the interpretation of metabolomic
data in mussels, as well as the impact of environmental stress in
marine invertebrates in general.
2014-06-17 00:00:00
1 H NMR spectroscopy
Female Mussels Exposed
seawater pH
1 H NMR Metabolomics
Reduced Seawater pH
marine
stress response impacting
OA
organism
challenge