Linshiz, Gregory Stawski, Nina Goyal, Garima Bi, Changhao Poust, Sean Sharma, Monica Mutalik, Vivek Keasling, Jay D. Hillson, Nathan J. PR-PR: Cross-Platform Laboratory Automation System To enable protocol standardization, sharing, and efficient implementation across laboratory automation platforms, we have further developed the PR-PR open-source high-level biology-friendly robot programming language as a cross-platform laboratory automation system. Beyond liquid-handling robotics, PR-PR now supports microfluidic and microscopy platforms, as well as protocol translation into human languages, such as English. While the same set of basic PR-PR commands and features are available for each supported platform, the underlying optimization and translation modules vary from platform to platform. Here, we describe these further developments to PR-PR, and demonstrate the experimental implementation and validation of PR-PR protocols for combinatorial modified Golden Gate DNA assembly across liquid-handling robotic, microfluidic, and manual platforms. To further test PR-PR cross-platform performance, we then implement and assess PR-PR protocols for Kunkel DNA mutagenesis and hierarchical Gibson DNA assembly for microfluidic and manual platforms. microscopy platforms;microfluidic;protocol standardization;Golden Gate DNA assembly;translation modules;Gibson DNA assembly;Kunkel DNA mutagenesis;protocol translation;laboratory automation platforms 2014-08-15
    https://acs.figshare.com/articles/dataset/PR_PR_Cross_Platform_Laboratory_Automation_System/2264518
10.1021/sb4001728.s002