%0 Generic %A Linshiz, Gregory %A Stawski, Nina %A Goyal, Garima %A Bi, Changhao %A Poust, Sean %A Sharma, Monica %A Mutalik, Vivek %A Keasling, Jay D. %A Hillson, Nathan J. %D 2014 %T PR-PR: Cross-Platform Laboratory Automation System %U https://acs.figshare.com/articles/dataset/PR_PR_Cross_Platform_Laboratory_Automation_System/2264518 %R 10.1021/sb4001728.s002 %2 https://acs.figshare.com/ndownloader/files/3900523 %K microscopy platforms %K microfluidic %K protocol standardization %K Golden Gate DNA assembly %K translation modules %K Gibson DNA assembly %K Kunkel DNA mutagenesis %K protocol translation %K laboratory automation platforms %X To enable protocol standardization, sharing, and efficient implementation across laboratory automation platforms, we have further developed the PR-PR open-source high-level biology-friendly robot programming language as a cross-platform laboratory automation system. Beyond liquid-handling robotics, PR-PR now supports microfluidic and microscopy platforms, as well as protocol translation into human languages, such as English. While the same set of basic PR-PR commands and features are available for each supported platform, the underlying optimization and translation modules vary from platform to platform. Here, we describe these further developments to PR-PR, and demonstrate the experimental implementation and validation of PR-PR protocols for combinatorial modified Golden Gate DNA assembly across liquid-handling robotic, microfluidic, and manual platforms. To further test PR-PR cross-platform performance, we then implement and assess PR-PR protocols for Kunkel DNA mutagenesis and hierarchical Gibson DNA assembly for microfluidic and manual platforms. %I ACS Publications