PR-PR: Cross-Platform Laboratory Automation System
Gregory Linshiz
Nina Stawski
Garima Goyal
Changhao Bi
Sean Poust
Monica Sharma
Vivek Mutalik
Jay D. Keasling
Nathan J. Hillson
10.1021/sb4001728.s002
https://acs.figshare.com/articles/dataset/PR_PR_Cross_Platform_Laboratory_Automation_System/2264518
To
enable protocol standardization, sharing, and efficient implementation
across laboratory automation platforms, we have further developed
the PR-PR open-source high-level biology-friendly robot programming
language as a cross-platform laboratory automation system. Beyond
liquid-handling robotics, PR-PR now supports microfluidic and microscopy
platforms, as well as protocol translation into human languages, such
as English. While the same set of basic PR-PR commands and features
are available for each supported platform, the underlying optimization
and translation modules vary from platform to platform. Here, we describe
these further developments to PR-PR, and demonstrate the experimental
implementation and validation of PR-PR protocols for combinatorial
modified Golden Gate DNA assembly across liquid-handling robotic,
microfluidic, and manual platforms. To further test PR-PR cross-platform
performance, we then implement and assess PR-PR protocols for Kunkel
DNA mutagenesis and hierarchical Gibson DNA assembly for microfluidic
and manual platforms.
2014-08-15 00:00:00
microscopy platforms
microfluidic
protocol standardization
Golden Gate DNA assembly
translation modules
Gibson DNA assembly
Kunkel DNA mutagenesis
protocol translation
laboratory automation platforms