10.1021/bm5009876.s001 Leandro S. Blachechen Leandro S. Blachechen Pedro Fardim Pedro Fardim Denise F. S. Petri Denise F. S. Petri Multifunctional Cellulose Beads and Their Interaction with Gram Positive Bacteria American Chemical Society 2014 bactericidal agent CHN XPS OCB samples UV HCl 2 M coagulation bath BacteriaCellulose beads charge density SEM Notorious bactericidal action scanning electron microscopy Multifunctional Cellulose Beads compression tests images analyses beads surface Carboxylic groups 2014-09-08 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Multifunctional_Cellulose_Beads_and_Their_Interaction_with_Gram_Positive_Bacteria/2256958 Cellulose beads with ∼3 mm of diameter and high circularity were obtained by dripping cellulose solutions (5, 6, and 7 wt %) dissolved in NaOH7%/urea12%, into HCl 2 M coagulation bath. Carboxylic groups were generated on beads surface through NaClO/NaClO<sub>2</sub>/TEMPO oxidation method, achieving total charge density of ∼0.77 mmol/g. Pristine (CB) and oxidized (OCB) beads were characterized by means of optical images analyses, scanning electron microscopy (SEM) and compression tests. Both types of beads, CB and OCB, were used as adsorbent for poly­(4-vinyl-<i>N</i>-pentylpyridinium) bromide, QPVP-C5, a bactericidal agent. The adsorption of QPVP-C5 on CB and OCB was evaluated by means of FTIR-ATR, UV–vis, CHN elemental analyses, and X-ray photoelectron spectroscopy (XPS). The adsorbed amount of QPVP-C5 was remarkably higher on OCB than on CB due to ionic interactions. Desorption was less than 5%. The interaction between neat OCB or OCB coated and two different amounts of QPVP-C5 and Gram-positive bacteria Micrococcus luteus was assessed by changes in turbidimetry, SEM, and elemental analyses. Bacteria adsorbed on the surface of neat OCB and weakly QPVP-C5 coated OCB due to hydrogen bonding or ion-dipole interaction. Notorious bactericidal action was observed for OCB samples coated with large amount of QPVP-C5.