10.1021/bm5009876.s001
Leandro
S. Blachechen
Leandro
S.
Blachechen
Pedro Fardim
Pedro
Fardim
Denise F. S. Petri
Denise
F. S. Petri
Multifunctional Cellulose Beads and Their Interaction
with Gram Positive Bacteria
American Chemical Society
2014
bactericidal agent
CHN
XPS
OCB samples
UV
HCl 2 M coagulation bath
BacteriaCellulose beads
charge density
SEM
Notorious bactericidal action
scanning electron microscopy
Multifunctional Cellulose Beads
compression tests
images analyses
beads surface
Carboxylic groups
2014-09-08 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Multifunctional_Cellulose_Beads_and_Their_Interaction_with_Gram_Positive_Bacteria/2256958
Cellulose beads with ∼3 mm
of diameter and high circularity
were obtained by dripping cellulose solutions (5, 6, and 7 wt %) dissolved
in NaOH7%/urea12%, into HCl 2 M coagulation bath. Carboxylic groups
were generated on beads surface through NaClO/NaClO<sub>2</sub>/TEMPO
oxidation method, achieving total charge density of ∼0.77 mmol/g.
Pristine (CB) and oxidized (OCB) beads were characterized by means
of optical images analyses, scanning electron microscopy (SEM) and
compression tests. Both types of beads, CB and OCB, were used as adsorbent
for poly(4-vinyl-<i>N</i>-pentylpyridinium) bromide, QPVP-C5,
a bactericidal agent. The adsorption of QPVP-C5 on CB and OCB was
evaluated by means of FTIR-ATR, UV–vis, CHN elemental analyses,
and X-ray photoelectron spectroscopy (XPS). The adsorbed amount of
QPVP-C5 was remarkably higher on OCB than on CB due to ionic interactions.
Desorption was less than 5%. The interaction between neat OCB or OCB
coated and two different amounts of QPVP-C5 and Gram-positive bacteria Micrococcus luteus was assessed by changes in turbidimetry,
SEM, and elemental analyses. Bacteria adsorbed on the surface of neat
OCB and weakly QPVP-C5 coated OCB due to hydrogen bonding or ion-dipole
interaction. Notorious bactericidal action was observed for OCB samples
coated with large amount of QPVP-C5.