pH-Responsive PDMS‑<i>b</i>‑PDMAEMA
Micelles for Intracellular Anticancer Drug Delivery
Anja Car
Patric Baumann
Jason
T. Duskey
Mohamed Chami
Nico Bruns
Wolfgang Meier
10.1021/bm500919z.s001
https://acs.figshare.com/articles/journal_contribution/pH_Responsive_PDMS_i_b_i_PDMAEMA_Micelles_for_Intracellular_Anticancer_Drug_Delivery/2256955
A series of poly(dimethysiloxane)-<i>b</i>-poly(2-(dimethylamino)ethyl
methacrylate) (PDMS-<i>b</i>-PDMAEMA) block copolymers were
synthesized with atom transfer radical polymerization (ATRP). In aqueous
solution the polymers self-assembled into micelles with diameters
between 80 and 300 nm, with the ability to encapsulate DOX. The polymer
with the shortest PDMAEMA block (5 units) displayed excellent cell
viability, while micelles containing longer PDMAEMA block lengths
(13 and 22 units) led to increased cytotoxicity. The carriers released
DOX in response to a decrease in pH from 7.4 to 5.5. Confocal laser
scanning microscopy (CLSM) revealed that nanoparticles were taken
up by endocytosis into acidic cell compartments. Furthermore, DOX-loaded
nanocarriers exhibited intracellular pH-response as changes in cell
morphology and drug release were observed within 24 h.
2014-09-08 00:00:00
DOX
PDMAEMA
drug release
Intracellular Anticancer Drug DeliveryA series
CLSM
300 nm
24 h
micelle
ATRP
block copolymers
PDMS
22 units
atom transfer
cell viability
cell morphology
5.5. Confocal laser scanning microscopy
acidic cell compartments