Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein–Protein Binding Affinities Rubén Ahijado-Guzmán Janak Prasad Christina Rosman Andreas Henkel Lydia Tome Dirk Schneider Germán Rivas Carsten Sönnichsen 10.1021/nl501865p.s001 https://acs.figshare.com/articles/journal_contribution/Plasmonic_Nanosensors_for_Simultaneous_Quantification_of_Multiple_Protein_Protein_Binding_Affinities/2247757 Most of current techniques used for the quantification of protein–protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories. 2014-10-08 00:00:00 Plasmonic Nanosensors binding affinities laboratory quantification protein Multiple technique binding partners Simultaneous Quantification affinity tool NanoSPR method nanorod interaction AffinitiesMost Binding Herein analysis spectroscopy Protein