Plasmonic Nanosensors for Simultaneous Quantification
of Multiple Protein–Protein Binding Affinities
Rubén Ahijado-Guzmán
Janak Prasad
Christina Rosman
Andreas Henkel
Lydia Tome
Dirk Schneider
Germán Rivas
Carsten Sönnichsen
10.1021/nl501865p.s001
https://acs.figshare.com/articles/journal_contribution/Plasmonic_Nanosensors_for_Simultaneous_Quantification_of_Multiple_Protein_Protein_Binding_Affinities/2247757
Most
of current techniques used for the quantification of protein–protein
interactions require the analysis of one pair of binding partners
at a time. Herein we present a label-free, simple, fast, and cost-effective
route to characterize binding affinities between multiple macromolecular
partners simultaneously, using optical dark-field spectroscopy and
individual protein-functionalized gold nanorods as sensing elements.
Our NanoSPR method could easily become a simple and standard tool
in biological, biochemical, and medical laboratories.
2014-10-08 00:00:00
Plasmonic Nanosensors
binding affinities
laboratory
quantification
protein
Multiple
technique
binding partners
Simultaneous Quantification
affinity
tool
NanoSPR method
nanorod
interaction
AffinitiesMost
Binding
Herein
analysis
spectroscopy
Protein