%0 Journal Article
%A Huang, Chun-Jen
%A Wang, Lin-Chuan
%A Shyue, Jing-Jong
%A Chang, Ying-Chih
%D 2014
%T Developing Antifouling Biointerfaces Based on Bioinspired
Zwitterionic Dopamine through pH-Modulated Assembly
%U https://acs.figshare.com/articles/journal_contribution/Developing_Antifouling_Biointerfaces_Based_on_Bioinspired_Zwitterionic_Dopamine_through_pH_Modulated_Assembly/2241124
%R 10.1021/la503191b.s001
%2 https://acs.figshare.com/ndownloader/files/3877063
%K zwitterionic sulfobetaine moiety
%K antifouling properties
%K pH transition approach
%K pH transition
%K TiO 2 converts
%K quartz crystal microbalance
%K contact angle goniometer
%K surface
%K adsorption
%K Bioinspired Zwitterionic Dopamine
%X The
use of synthetic biomaterials as implantable devices typically is
accompanied by considerable nonspecific adsorption of proteins, cells,
and bacteria. These may eventually induce adverse pathogenic problems
in clinical practice, such as thrombosis and biomaterial-associated
infection. Thus, an effective surface coating for medical devices
has been pursued to repel nonspecific adsorption from surfaces. In
this study, we employ an adhesive dopamine molecule conjugated with
zwitterionic sulfobetaine moiety (SB-DA), developed based on natural
mussels, as a surface ligand for the modification of TiO2. The electrochemical study shows that the SB-DA exhibits fully reversible
reduction–oxidation behavior at pH 3, but it is irreversible
at pH 8. A contact angle goniometer and X-ray photoelectron spectroscopy
were utilized to explore the surface hydration, chemical states, and
bonding mechanism of SB-DA. The results indicate that the binding
between hydroxyl groups of SB-DA and TiO2 converts from
hydrogen bonds to bidentate binding upon the pH transition from pH
3 to 8. In order to examine the antifouling properties of SB-DA thin
films, the modified substrates were brought into contact with bovine
serum albumin and bacteria solutions. The fouling levels were monitored
using a quartz crystal microbalance with dissipation sensor and fluorescence
optical microscope. Tests showed that the sample prepared via the
pH transition approach provides the best resistance to nonspecific
adsorption due to the high coverage and stability of the SB-DA films.
These findings support the mechanism of the pH-modulated assembly
of SB-DA molecules, and for the first time we demonstrate the antifouling
properties of the SB-DA to be comparable with traditional thiol-based
zwitterionic self-assemblies. The success of modification with SB-DA
opens an avenue for developing a biologically inspired surface chemistry
and can have applications over a wide spectrum of bioapplications.
The strategy of the pH transition can also be applied to other functional
dopamine derivatives.
%I ACS Publications