10.1021/pr5006789.s010
Veneta Qendro
Veneta
Qendro
Deborah
H. Lundgren
Deborah
H.
Lundgren
Karim Rezaul
Karim
Rezaul
Forrest Mahony
Forrest
Mahony
Nicholas Ferrell
Nicholas
Ferrell
Andrew Bi
Andrew
Bi
Ardian Latifi
Ardian
Latifi
Daniyal Chowdhury
Daniyal
Chowdhury
Steven Gygi
Steven
Gygi
Wilhelm Haas
Wilhelm
Haas
Lori Wilson
Lori
Wilson
Michael Murphy
Michael
Murphy
David K. Han
David K.
Han
Large-Scale Proteomic Characterization
of Melanoma
Expressed Proteins Reveals Nestin and Vimentin as Biomarkers That
Can Potentially Distinguish Melanoma Subtypes
American Chemical Society
2014
literature mining
tandem mass spectrometry
protein biomarkers
melanoma cell lines
research community
melanoma subtypes
metastatic melanoma tumor
skin cancer cases
melanoma aggressiveness
40 melanoma samples
histone H 2A Validation
skin cancer deaths
melanoma biomarkers
skin cancer
Distinguish Melanoma SubtypesMelanoma
Correlating protein expression
IV
melanoma cell line
protein expression
2014-11-07 00:00:00
Dataset
https://acs.figshare.com/articles/dataset/Large_Scale_Proteomic_Characterization_of_Melanoma_Expressed_Proteins_Reveals_Nestin_and_Vimentin_as_Biomarkers_That_Can_Potentially_Distinguish_Melanoma_Subtypes/2238583
Melanoma
is an aggressive type of skin cancer, which accounts for
only 4% of skin cancer cases but causes around 75% of skin cancer
deaths. Currently, there is a limited set of protein biomarkers that
can distinguish melanoma subtypes and provide an accurate prognosis
of melanoma. Thus, we have selected and profiled the proteomes of
five different melanoma cell lines from different stages of progression
in comparison with a normal melanocytes using tandem mass spectrometry.
We also profiled the proteome of a solid metastatic melanoma tumor.
This resulted in the identification of 4758 unique proteins, among
which ∼200–300 differentially expressed proteins from
each set were found by quantitative proteomics. Correlating protein
expression with aggressiveness of each melanoma cell line and literature
mining resulted in the final selection of six proteins: vimentin,
nestin, fibronectin, annexin A1, dipeptidyl peptidase IV, and histone
H2A1B. Validation of nestin and vimentin using 40 melanoma samples
revealed pattern of protein expression can help predict melanoma aggressiveness
in different subgroups of melanoma. These results, together with the
combined list of 4758 expressed proteins, provide a valuable resource
for selecting melanoma biomarkers in the future for the clinical and
research community.