10.1021/la504558z.s001
Urszula Bazylińska
Urszula
Bazylińska
Sławomir Drozdek
Sławomir
Drozdek
Marcin Nyk
Marcin
Nyk
Julita Kulbacka
Julita
Kulbacka
Marek Samoć
Marek
Samoć
Kazimiera A. Wilk
Kazimiera A.
Wilk
Core/Shell Quantum Dots Encapsulated in Biocompatible
Oil-Core Nanocarriers as Two-Photon Fluorescent Markers for Bioimaging
American Chemical Society
2014
Cremophor EL
oil phases
Poloxamer 403
CdSe
silicone oil
quantum dots
MCF
GM
alveolar basal epithelial
NIR
core
cell imaging
polymer component
nanocapsules exhibit
encapsulated QDs
800 nm
particle size
luminescence properties
transmission electron microscopy
CLSM
HUVEC
bioimaging application
cytotoxicity studies
umbilical vein
confocal laser scanning microscopy
force microscopy
excitation
oleic acid
transmission window
intracellular localization
mineral oil
2014-12-16 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Core_Shell_Quantum_Dots_Encapsulated_in_Biocompatible_Oil_Core_Nanocarriers_as_Two_Photon_Fluorescent_Markers_for_Bioimaging/2225176
Highly fluorescent quantum dots (QDs)-loaded
nanocapsules, intended
for fluorescent cell imaging, were prepared via an emulsification/solvent-evaporation
method. CdSe/ZnS core/shell quantum dots were applied as cargo; Poloxamer
403 as the polymer component; Cremophor EL as the nonionic surfactant;
and mineral oil, oleic acid, or silicone oil were applied as the oil
phases. Transmission electron microscopy, atomic force microscopy,
dynamic light scattering, and zeta potential measurements were used
to characterize the novel QDs-labeled nanoparticles by particle size,
distribution, and morphology, as well as by ζ-potential and
physical stability. The fabricated long-lasting nanocapsules exhibit
good luminescence properties upon both one-photon and two-photon excitation.
The potential of the encapsulated QDs for fluorescent imaging was
evaluated in cytotoxicity studies as well as in imaging of intracellular
localization, accumulation, and distribution of QDs delivered to well-characterized
human cancer cell linesdoxorubicin-sensitive breast (MCF-7/WT)
and alveolar basal epithelial (A549)as well as on normal human
umbilical vein endothelial (HUVEC) cells, as investigated by confocal
laser scanning microscopy (CLSM). The colloidal CdSe/ZnS-loaded nanocapsules
are shown to exhibit strong two-photon-induced luminescence upon excitation
in the NIR optical transmission window spectral range, making them
ideal markers for bioimaging application. The total two-photon cross
section of a single nanocapsule was determined to be about 4.1 ×
10<sup>6</sup> GM at 800 nm.