10.1021/la504379v.s001
Svetlana E. Solovieva
Svetlana E.
Solovieva
Roman
A. Safiullin
Roman
A.
Safiullin
Evgeni N. Kochetkov
Evgeni N.
Kochetkov
Nina B. Melnikova
Nina B.
Melnikova
Marsil K. Kadirov
Marsil K.
Kadirov
Elena V. Popova
Elena V.
Popova
Igor S. Antipin
Igor S.
Antipin
Alexander I. Konovalov
Alexander I.
Konovalov
Langmuir Monolayers and Thin Films of Amphifilic Thiacalix[4]arenes.
Properties and Matrix for the Immobilization of Cytochrome <i>c</i>
American Chemical Society
2014
Langmuir films
OCH
Cytochrome cFormation
surface pressure
NH
immobilize cytochrome c
protein activity
Obtained monolayers
TCA
monomolecular layer
Langmuir Monolayers
ITO
substrate
Thin Films
2CN
2014-12-23 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Langmuir_Monolayers_and_Thin_Films_of_Amphifilic_Thiacalix_4_arenes_Properties_and_Matrix_for_the_Immobilization_of_Cytochrome_i_c_i_/2222926
Formation and properties
of Langmuir films of thiacalix[4]arene
(TCA) derivatives containing N-donor groups on the lower rim (YO(CH<sub>2</sub>)<sub>3</sub>CN; OCH<sub>2</sub>CN; NH<sub>2</sub>; OCH<sub>2</sub>ArCN-<i>p</i>) in <i>1</i>,<i>3</i>-<i>alternate</i> conformation on aqueous subphase and
solid substrates have been studied. Only <i>tetra</i>-cyanopropoxy-<i>p</i>-<i>tert</i>-butylthiacalix[4]arene <b>1</b> forms a typical monomolecular layer with perpendicular orientation
of the macrocycle relative to the water–air interface that
is able to immobilize cytochrome <i>c</i> in the entire
range of the surface pressure. Obtained monolayers were transferred
by Langmuir–Schaefer technique onto quartz, indium–tin
oxide (ITO), and silicon. It was demonstrated that protein activity
is retained after immobilization on the substrate.