10.1021/la504379v.s001 Svetlana E. Solovieva Svetlana E. Solovieva Roman A. Safiullin Roman A. Safiullin Evgeni N. Kochetkov Evgeni N. Kochetkov Nina B. Melnikova Nina B. Melnikova Marsil K. Kadirov Marsil K. Kadirov Elena V. Popova Elena V. Popova Igor S. Antipin Igor S. Antipin Alexander I. Konovalov Alexander I. Konovalov Langmuir Monolayers and Thin Films of Amphifilic Thiacalix[4]arenes. Properties and Matrix for the Immobilization of Cytochrome <i>c</i> American Chemical Society 2014 Langmuir films OCH Cytochrome cFormation surface pressure NH immobilize cytochrome c protein activity Obtained monolayers TCA monomolecular layer Langmuir Monolayers ITO substrate Thin Films 2CN 2014-12-23 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Langmuir_Monolayers_and_Thin_Films_of_Amphifilic_Thiacalix_4_arenes_Properties_and_Matrix_for_the_Immobilization_of_Cytochrome_i_c_i_/2222926 Formation and properties of Langmuir films of thiacalix[4]­arene (TCA) derivatives containing N-donor groups on the lower rim (YO­(CH<sub>2</sub>)<sub>3</sub>CN; OCH<sub>2</sub>CN; NH<sub>2</sub>; OCH<sub>2</sub>ArCN-<i>p</i>) in <i>1</i>,<i>3</i>-<i>alternate</i> conformation on aqueous subphase and solid substrates have been studied. Only <i>tetra</i>-cyanopropoxy-<i>p</i>-<i>tert</i>-butylthiacalix­[4]­arene <b>1</b> forms a typical monomolecular layer with perpendicular orientation of the macrocycle relative to the water–air interface that is able to immobilize cytochrome <i>c</i> in the entire range of the surface pressure. Obtained monolayers were transferred by Langmuir–Schaefer technique onto quartz, indium–tin oxide (ITO), and silicon. It was demonstrated that protein activity is retained after immobilization on the substrate.