10.1021/pr5006873.s009
Guozhang Kang
Guozhang
Kang
Gezi Li
Gezi
Li
Lina Wang
Lina
Wang
Liting Wei
Liting
Wei
Yang Yang
Yang
Yang
Pengfei Wang
Pengfei
Wang
Yingying Yang
Yingying
Yang
Yonghua Wang
Yonghua
Wang
Wei Feng
Wei
Feng
Chenyang Wang
Chenyang
Wang
Tiancai Guo
Tiancai
Guo
Hg-Responsive Proteins Identified
in Wheat Seedlings
Using iTRAQ Analysis and the Role of ABA in Hg Stress
American Chemical Society
2015
wheat protein response
Exogenous ABA application
Hg StressWheat seedlings
100 μ M HgCl 2
wheat seedlings
Pathway Studio software
Hg stress
2015-01-02 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Hg_Responsive_Proteins_Identified_in_Wheat_Seedlings_Using_iTRAQ_Analysis_and_the_Role_of_ABA_in_Hg_Stress/2220253
Wheat
seedlings exposed to 100 μM HgCl<sub>2</sub> for 3
days exhibited high-level mercury (Hg) accumulation, which led to
inhibited growth, increased lipid peroxidation, and disrupted cellular
ultrastructures. And root growth and ultrastructural changes of wheat
seedlings were inhibited more severely than those of leaves. To identify
the wheat protein response to Hg stress, the iTRAQ method was used
to determine the proteome profiles of the roots and leaves of wheat
seedlings exposed to high-Hg conditions. 249 proteins were identified
with significantly altered abundance. 117 were found in roots and
132 in leaves. These proteins were classified into signal transduction,
stress defense, carbohydrate metabolism, protein metabolism, energy
production, and transport functional groups. The majority of proteins
identified in Hg-stressed roots and leaves displayed differently altered
abundance, revealing organ-specific differences in adaption to Hg
stress. Pathway Studio software was used to identify the Hg-responsive
protein interaction network that included 49 putative key proteins,
and they were potentially regulated by abscisic acid (ABA). Exogenous
ABA application conferred protection against Hg stress and increased
activities of peroxidase enzyme, suggesting that it may be an important
factor in the Hg signaling pathway. These findings can provide useful
insights into the molecular mechanisms of Hg responses in higher plants.