10.1021/pr5006873.s009 Guozhang Kang Guozhang Kang Gezi Li Gezi Li Lina Wang Lina Wang Liting Wei Liting Wei Yang Yang Yang Yang Pengfei Wang Pengfei Wang Yingying Yang Yingying Yang Yonghua Wang Yonghua Wang Wei Feng Wei Feng Chenyang Wang Chenyang Wang Tiancai Guo Tiancai Guo Hg-Responsive Proteins Identified in Wheat Seedlings Using iTRAQ Analysis and the Role of ABA in Hg Stress American Chemical Society 2015 wheat protein response Exogenous ABA application Hg StressWheat seedlings 100 μ M HgCl 2 wheat seedlings Pathway Studio software Hg stress 2015-01-02 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Hg_Responsive_Proteins_Identified_in_Wheat_Seedlings_Using_iTRAQ_Analysis_and_the_Role_of_ABA_in_Hg_Stress/2220253 Wheat seedlings exposed to 100 μM HgCl<sub>2</sub> for 3 days exhibited high-level mercury (Hg) accumulation, which led to inhibited growth, increased lipid peroxidation, and disrupted cellular ultrastructures. And root growth and ultrastructural changes of wheat seedlings were inhibited more severely than those of leaves. To identify the wheat protein response to Hg stress, the iTRAQ method was used to determine the proteome profiles of the roots and leaves of wheat seedlings exposed to high-Hg conditions. 249 proteins were identified with significantly altered abundance. 117 were found in roots and 132 in leaves. These proteins were classified into signal transduction, stress defense, carbohydrate metabolism, protein metabolism, energy production, and transport functional groups. The majority of proteins identified in Hg-stressed roots and leaves displayed differently altered abundance, revealing organ-specific differences in adaption to Hg stress. Pathway Studio software was used to identify the Hg-responsive protein interaction network that included 49 putative key proteins, and they were potentially regulated by abscisic acid (ABA). Exogenous ABA application conferred protection against Hg stress and increased activities of peroxidase enzyme, suggesting that it may be an important factor in the Hg signaling pathway. These findings can provide useful insights into the molecular mechanisms of Hg responses in higher plants.