10.1021/acs.analchem.5b00135.s001
Qian Chen
Qian
Chen
Han Wang
Han
Wang
Hui Liu
Hui
Liu
Shihui Wen
Shihui
Wen
Chen Peng
Chen
Peng
Mingwu Shen
Mingwu
Shen
Guixiang Zhang
Guixiang
Zhang
Xiangyang Shi
Xiangyang
Shi
Multifunctional Dendrimer-Entrapped Gold Nanoparticles
Modified with RGD Peptide for Targeted Computed Tomography/Magnetic
Resonance Dual-Modal Imaging of Tumors
American Chemical Society
2015
multifunctional dendrimers
Gd chelator
PEG monomethyl ether
dendrimer terminal amines
DENP
imaging
MR
100 μ M
RGD Peptide
TumorsWe report
CT
3.8 nm
tumor model
entrap gold nanoparticles
polyethylene glycol
core size
2015-04-07 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Multifunctional_Dendrimer_Entrapped_Gold_Nanoparticles_Modified_with_RGD_Peptide_for_Targeted_Computed_Tomography_Magnetic_Resonance_Dual_Modal_Imaging_of_Tumors/2179267
We
report the use of multifunctional dendrimer-entrapped gold nanoparticles
(Au DENPs) loaded with gadolinium (Gd) chelator/Gd(III) complexes
and surface-modified with thiolated cyclo(Arg-Gly-Asp-Phe-Lys(mpa))
(RGD) peptide for targeted dual-mode computed tomography (CT)/magnetic
resonance (MR) imaging of small tumors. In this study, amine-terminated
generation 5 poly(amidoamine) dendrimers were used as a nanoplatform
to be covalently modified with Gd chelator, RGD via a polyethylene
glycol (PEG) spacer, and PEG monomethyl ether. Then the multifunctional
dendrimers were used as templates to entrap gold nanoparticles, followed
by chelating Gd(III) ions and acetylation of the remaining dendrimer
terminal amines. The thus-formed multifunctional Au DENPs (in short,
Gd–Au DENPs-RGD) were characterized via different techniques.
We show that the multifunctional Au DENPs with a Au core size of 3.8
nm are water-dispersible, stable under different pH (5–8) and
temperature conditions (4–50 °C), and noncytotoxic at
a Au concentration up to 100 μM. With the displayed X-ray attenuation
property and the <i>r</i><sub>1</sub> relaxivity (2.643
mM<sup>–1</sup> s<sup>–1</sup>), the developed Gd–Au
DENPs-RGD are able to be used as a dual-mode nanoprobe for targeted
CT/MR imaging of an α<sub><i>v</i></sub>β<sub>3</sub> integrin-overexpressing xenografted small tumor model in
vivo via RGD-mediated active targeting pathway. The developed multifunctional
Gd–Au DENPs-RGD may be used as a promising dual-mode nanoprobe
for targeted CT/MR imaging of different types of α<sub><i>v</i></sub>β<sub>3</sub> integrin-overexpressing cancer.