10.1021/acs.est.5b01244.s001
Jeonghoon Han
Jeonghoon
Han
Eun-Ji Won
Eun-Ji
Won
Hui-Su Kim
Hui-Su
Kim
David
R. Nelson
David
R.
Nelson
Su-Jae Lee
Su-Jae
Lee
Heum Gi Park
Heum Gi
Park
Jae-Seong Lee
Jae-Seong
Lee
Identification
of the Full 46 Cytochrome P450 (<i>CYP</i>) Complement and
Modulation of <i>CYP</i> Expression
in Response to Water-Accommodated Fractions of Crude Oil in the Cyclopoid Copepod <i>Paracyclopina
nana</i>
American Chemical Society
2015
PAH
WAF exposure
nana CYP gene superfamily
marine copepod Paracyclopina nana
Full 46 Cytochrome P 450
CYP 3027F
Cyclopoid Copepod Paracyclopina nanaThe 46 cytochrome P 450
CYP genes
response
copepod CYP gene families
copepod Tigriopus japonicus
2015-06-02 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Identification_of_the_Full_46_Cytochrome_P450_i_CYP_i_Complement_and_Modulation_of_i_CYP_i_Expression_in_Response_to_Water_Accommodated_Fractions_of_Crude_Oil_in_the_Cyclopoid_Copepod_i_Paracyclopina_nana_i_/2162779
The 46 cytochrome P450 (<i>CYP</i>) gene superfamily
was identified in the marine copepod <i>Paracyclopina nana</i> after searching an RNA-seq database and comparing it with other
copepod <i>CYP</i> gene families. To annotate the 46 <i>Pn-CYP</i> genes, a phylogenetic analysis of <i>CYP</i> genes was performed using a Bayesian method. <i>Pn-CYP</i> genes were separated into five different clans: CYP2, CYP3, CYP20,
CYP26, and mitochondrial. Among these, the principal <i>Pn-CYP</i> genes involved in detoxification were identified by comparing them
with those of the copepod <i>Tigriopus japonicus</i> and
were examined with respect to their responses to exposure to a water-accommodated
fraction (WAF) of crude oil and to the alkylated forms of two polycyclic
aromatic hydrocarbons (PAHs; phenanthrene and fluorene). The expression
of two <i>Pn-CYP3027</i> genes (<i>CYP3027F1</i> and <i>CYP3027F2</i>) was increased in response to WAF
exposure and also was upregulated in response to the two alkylated
PAHs. In particular, <i>Pn-CYP3027F2</i> showed the most
notable increase in response to 80% WAF exposure. These two responsive <i>CYP</i> genes (<i>Pn-CYP3027F1</i> and <i>CYP3027F2</i>) were also phylogenetically clustered into the same clade of the
WAF- and alkylated PAH-specific <i>CYP</i> genes of the
copepod <i>T. japonicus</i>, suggesting that these <i>CYP</i> genes would be those chiefly involved in detoxification
in response to WAF exposure in copepods. In this paper, we provide
information on the copepod <i>P. nana CYP</i> gene superfamily
and also speculate on its potential role in the detoxification of
PAHs in marine copepods. Despite the nonlethality of WAF, <i>Pn-CYP3027F2</i> was rapidly and significantly upregulated in
response to WAF that may serve as a useful biomarker of 40% or higher
concentration of WAF exposure. This paper will be helpful to better
understand the molecular mechanistic events underlying the metabolism
of environmental toxicants in copepods.