10.1021/acs.analchem.5b02383.s001 Eden Morales-Narváez Eden Morales-Narváez Tina Naghdi Tina Naghdi Erhan Zor Erhan Zor Arben Merkoçi Arben Merkoçi Photoluminescent Lateral-Flow Immunoassay Revealed by Graphene Oxide: Highly Sensitive Paper-Based Pathogen Detection American Chemical Society 2015 Immunoassay Escherichia coli Photoluminescent Pathogen graphene oxide performance Revealed mL Sensitive flow immunoassay device pathogen detection DetectionA model pathogen photoluminescence quenching ability Graphene Oxide diagnostics applications CFU antibody display 2015-08-18 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Photoluminescent_Lateral_Flow_Immunoassay_Revealed_by_Graphene_Oxide_Highly_Sensitive_Paper_Based_Pathogen_Detection/2140195 A paper-based lateral flow immunoassay for pathogen detection that avoids the use of secondary antibodies and is revealed by the photoluminescence quenching ability of graphene oxide is reported. <i>Escherichia coli</i> has been selected as a model pathogen. The proposed device is able to display a highly specific and sensitive performance with a limit of detection of 10 CFU mL<sup>–1</sup> in standard buffer and 100 CFU mL<sup>–1</sup> in bottled water and milk. This low-cost disposable and easy-to-use device will prove valuable for portable and automated diagnostics applications.