10.1021/acs.langmuir.5b02004.s001 Amir Landarani-Isfahani Amir Landarani-Isfahani Asghar Taheri-Kafrani Asghar Taheri-Kafrani Mina Amini Mina Amini Valiollah Mirkhani Valiollah Mirkhani Majid Moghadam Majid Moghadam Asieh Soozanipour Asieh Soozanipour Amir Razmjou Amir Razmjou Xylanase Immobilized on Novel Multifunctional Hyperbranched Polyglycerol-Grafted Magnetic Nanoparticles: An Efficient and Robust Biocatalyst American Chemical Society 2015 Robust BiocatalystAlthough immobilization hyperbranched polyglycerol hyperbranched polymers chemical properties strategy enzyme biocatalyst systems xylanase MNP nanoparticle Xylanase Immobilized 2015-08-25 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Xylanase_Immobilized_on_Novel_Multifunctional_Hyperbranched_Polyglycerol_Grafted_Magnetic_Nanoparticles_An_Efficient_and_Robust_Biocatalyst/2137975 Although several strategies are now available for immobilization of enzymes to magnetic nanoparticles for bioapplications, little progresses have been reported on the use of dendritic or hyperbranched polymers for the same purpose. Herein, we demonstrated synthesis of magnetic nanoparticles supported hyperbranched polyglycerol (MNP/HPG) and a derivative conjugated with citric acid (MNP/HPG-CA) as unique and convenient nanoplatforms for immobilization of enzymes. Then, an important industrial enzyme, xylanase, was immobilized on the nanocarriers to produce robust biocatalysts. A variety of analytical tools were used to study the morphological, structural, and chemical properties of the biocatalysts. Additionally, the results of biocatalyst systems exhibited the substantial improvement of reactivity, reusability, and stability of xylanase due to this strategy, which might confer them a wider range of applications.