Zhang, Jianjian Ning, Lulu Liu, Jiting Wang, Jianxi Yu, Bianfei Liu, Xiaoyan Yao, Xiaojun Zhang, Ziping Zhang, Haixia Naked-Eye and Near-Infrared Fluorescence Probe for Hydrazine and Its Applications in In Vitro and In Vivo Bioimaging Hydrazine has been applied diffusely in most of the chemical industry; however, it is a hazardous environmental pollutant and highly toxic to organisms. Selective, rapid, and sensitive detection of hydrazine thus becomes absolutely necessary in both biological and environmental sciences. Accordingly, fluorescence probes for hydrazine have been paid great attention in recent years. Disclosed here is the near-infrared (NIR) fluorescence probe with a turn-on fluorescent probe <b>CyJ</b> based on the structure–emission property relationships of the NIR dyes containing an acetyl group as the recognizing moiety. This new probe not only can be readily prepared, but also shows excellent sensing properties. First and most important of all, <b>CyJ</b> is highly selective for N<sub>2</sub>H<sub>4</sub> over various anions, cations, and other amino compounds and has a low limit of detection (LOD) of hydrazine (5.4 ppb as fluorescence sensor and 6.1 ppb as UV sensor). Besides, <b>CyJ</b> exhibited a dramatic increase in fluorescence at λ<sub>max</sub> = 706 nm in the presence of N<sub>2</sub>H<sub>4</sub>, and it offers a rapid, colorimetric and vapor sensing detection process for N<sub>2</sub>H<sub>4</sub> in both aqueous solution and diluted human serum. Furthermore, <b>CyJ</b> has good cell-membrane permeability and low cytotoxicity. In addition, we have successfully applied the <b>CyJ</b> to visualize N<sub>2</sub>H<sub>4</sub> in live mouse and, for the first time, in tissues such as the liver, lung, kidney, heart, and spleen. probe;CyJ;N 2H;NIR;detection;sensor;UV;ppb;hydrazine;LOD;fluorescence 2015-09-01
    https://acs.figshare.com/articles/journal_contribution/Naked_Eye_and_Near_Infrared_Fluorescence_Probe_for_Hydrazine_and_Its_Applications_in_In_Vitro_and_In_Vivo_Bioimaging/2136493
10.1021/acs.analchem.5b02527.s001