10.1021/acs.jafc.5b02108.s001 Iqbal Mahmud Iqbal Mahmud Chandrasekar Kousik Chandrasekar Kousik Richard Hassell Richard Hassell Kamal Chowdhury Kamal Chowdhury Arezue F. Boroujerdi Arezue F. Boroujerdi NMR Spectroscopy Identifies Metabolites Translocated from Powdery Mildew Resistant Rootstocks to Susceptible Watermelon Scions American Chemical Society 2015 rootstock Mickey Lee Powdery Mildew Resistant Rootstocks multivariate loading plots metabolite Susceptible Watermelon ScionsPowdery mildew Mahalanobis distance analysis volcano plot analyses PM resistance scion component analysis NMR Spectroscopy Identifies Metabolites Translocated Significant score plot differences disease causes PM disease resistance watermelon variety management strategy 2015-09-16 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/NMR_Spectroscopy_Identifies_Metabolites_Translocated_from_Powdery_Mildew_Resistant_Rootstocks_to_Susceptible_Watermelon_Scions/2131390 Powdery mildew (PM) disease causes significant loss in watermelon. Due to the unavailability of a commercial watermelon variety that is resistant to PM, grafting susceptible cultivars on wild resistant rootstocks is being explored as a short-term management strategy to combat this disease. Nuclear magnetic resonance-based metabolic profiles of susceptible and resistant rootstocks of watermelon and their corresponding susceptible scions (Mickey Lee) were compared to screen for potential metabolites related to PM resistance using multivariate principal component analysis. Significant score plot differences between the susceptible and resistant groups were revealed through Mahalanobis distance analysis. Significantly different spectral buckets and their corresponding metabolites (including choline, fumarate, 5-hydroxyindole-3-acetate, and melatonin) have been identified quantitatively using multivariate loading plots and verified by volcano plot analyses. The data suggest that these metabolites were translocated from the powdery mildew resistant rootstocks to their corresponding powdery mildew susceptible scions and can be related to PM disease resistance.