%0 Journal Article
%A Liu, Qingpo
%A Hu, Haichao
%A Zhu, Leyi
%A Li, Ruochen
%A Feng, Ying
%A Zhang, Liqing
%A Yang, Yuyan
%A Liu, Xingquan
%A Zhang, Hengmu
%D 2015
%T Involvement of miR528 in the Regulation of Arsenite
Tolerance in Rice (Oryza sativa L.)
%U https://acs.figshare.com/articles/journal_contribution/Involvement_of_miR528_in_the_Regulation_of_Arsenite_Tolerance_in_Rice_Oryza_sativa_L_/2122168
%R 10.1021/acs.jafc.5b04191.s004
%2 https://acs.figshare.com/ndownloader/files/3755977
%K miR 528
%K acid profiles
%K acid content changes
%K oxidative stress generation
%K target genes
%K stress conditions
%K expression profiles
%K Arsenite Tolerance
%K Ubi
%K WT plants
%K antioxidant enzyme activity
%K tolerance systems
%K plants overexpressing miR 528
%X Tens of miRNAs were previously established
as being arsenic (As)
stress responsive in rice. However, their functional role in As tolerance
remains unclear. This study demonstrates that transgenic plants overexpressing
miR528 (Ubi::MIR528) were more sensitive to arsenite
[As(III)] compared with wild-type (WT) rice. Under normal and stress
conditions, miR528-5p and -3p were highly up-regulated in both the
roots and leaves of transgenic plants, which exhibited a negative
correlation with the expression of seven target genes. Compared with
WT plants, Ubi::MIR528 plants showed excessive oxidative
stress generation and remarkable amino acid content changes in the
roots and leaves upon As(III) exposure. Notably, the expression profiles
of diverse functional genes were clearly different between WT and
transgenic plants. Thus, the observed As(III) sensitivity of Ubi::MIR528 plants was likely due to the strong alteration
of antioxidant enzyme activity and amino acid profiles and the impairment
of the As(III) uptake, translocation, and tolerance systems of rice.
%I ACS Publications