10.1021/acs.jafc.5b04191.s004 Qingpo Liu Qingpo Liu Haichao Hu Haichao Hu Leyi Zhu Leyi Zhu Ruochen Li Ruochen Li Ying Feng Ying Feng Liqing Zhang Liqing Zhang Yuyan Yang Yuyan Yang Xingquan Liu Xingquan Liu Hengmu Zhang Hengmu Zhang Involvement of miR528 in the Regulation of Arsenite Tolerance in Rice (Oryza sativa L.) American Chemical Society 2015 miR 528 acid profiles acid content changes oxidative stress generation target genes stress conditions expression profiles Arsenite Tolerance Ubi WT plants antioxidant enzyme activity tolerance systems plants overexpressing miR 528 2015-10-14 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Involvement_of_miR528_in_the_Regulation_of_Arsenite_Tolerance_in_Rice_Oryza_sativa_L_/2122168 Tens of miRNAs were previously established as being arsenic (As) stress responsive in rice. However, their functional role in As tolerance remains unclear. This study demonstrates that transgenic plants overexpressing miR528 (<i>Ubi::MIR528</i>) were more sensitive to arsenite [As­(III)] compared with wild-type (WT) rice. Under normal and stress conditions, miR528-5p and -3p were highly up-regulated in both the roots and leaves of transgenic plants, which exhibited a negative correlation with the expression of seven target genes. Compared with WT plants, <i>Ubi::MIR528</i> plants showed excessive oxidative stress generation and remarkable amino acid content changes in the roots and leaves upon As­(III) exposure. Notably, the expression profiles of diverse functional genes were clearly different between WT and transgenic plants. Thus, the observed As­(III) sensitivity of <i>Ubi::MIR528</i> plants was likely due to the strong alteration of antioxidant enzyme activity and amino acid profiles and the impairment of the As­(III) uptake, translocation, and tolerance systems of rice.