10.1021/acs.jafc.5b04191.s004
Qingpo Liu
Qingpo
Liu
Haichao Hu
Haichao
Hu
Leyi Zhu
Leyi
Zhu
Ruochen Li
Ruochen
Li
Ying Feng
Ying
Feng
Liqing Zhang
Liqing
Zhang
Yuyan Yang
Yuyan
Yang
Xingquan Liu
Xingquan
Liu
Hengmu Zhang
Hengmu
Zhang
Involvement of miR528 in the Regulation of Arsenite
Tolerance in Rice (Oryza sativa L.)
American Chemical Society
2015
miR 528
acid profiles
acid content changes
oxidative stress generation
target genes
stress conditions
expression profiles
Arsenite Tolerance
Ubi
WT plants
antioxidant enzyme activity
tolerance systems
plants overexpressing miR 528
2015-10-14 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Involvement_of_miR528_in_the_Regulation_of_Arsenite_Tolerance_in_Rice_Oryza_sativa_L_/2122168
Tens of miRNAs were previously established
as being arsenic (As)
stress responsive in rice. However, their functional role in As tolerance
remains unclear. This study demonstrates that transgenic plants overexpressing
miR528 (<i>Ubi::MIR528</i>) were more sensitive to arsenite
[As(III)] compared with wild-type (WT) rice. Under normal and stress
conditions, miR528-5p and -3p were highly up-regulated in both the
roots and leaves of transgenic plants, which exhibited a negative
correlation with the expression of seven target genes. Compared with
WT plants, <i>Ubi::MIR528</i> plants showed excessive oxidative
stress generation and remarkable amino acid content changes in the
roots and leaves upon As(III) exposure. Notably, the expression profiles
of diverse functional genes were clearly different between WT and
transgenic plants. Thus, the observed As(III) sensitivity of <i>Ubi::MIR528</i> plants was likely due to the strong alteration
of antioxidant enzyme activity and amino acid profiles and the impairment
of the As(III) uptake, translocation, and tolerance systems of rice.