%0 Journal Article
%A Wang, Reuben
%A Lin, Pei-Yin
%A Huang, Shyue-Tsong
%A Chiu, Chun-Hui
%A Lu, Ting-Jang
%A Lo, Yi-Chen
%D 2015
%T Hyperproduction of β‑Glucanase Exg1 Promotes
the Bioconversion of Mogrosides in Saccharomyces cerevisiae Mutants Defective in Mannoprotein Deposition
%U https://acs.figshare.com/articles/journal_contribution/Hyperproduction_of_Glucanase_Exg1_Promotes_the_Bioconversion_of_Mogrosides_in_Saccharomyces_cerevisiae_Mutants_Defective_in_Mannoprotein_Deposition/2104219
%R 10.1021/acs.jafc.5b03909.s001
%2 https://acs.figshare.com/ndownloader/files/3737485
%K mogroside III E
%K secrete extracellular enzymes
%K MG
%K Saccharomyces cerevisiae Mutants Defective
%K zymolyase sensitivity test
%K Saccharomyces cerevisiae kre 6Δ mutants
%K transmission electron microscopy
%K cell wall structure
%K glycosylated bioactive compounds
%K mannoprotein deposition
%K mannoprotein release assay
%X Bacteria
and fungi can secrete extracellular enzymes to convert
macromolecules into smaller units. Hyperproduction of extracellular
enzymes is often associated with alterations in cell wall structure
in fungi. Recently, we identified that Saccharomyces
cerevisiae kre6Δ mutants can
efficiently convert mogroside V into mogroside III E, which has antidiabetic
properties. However, the underlying efficient bioconversion mechanism
is unclear. In the present
study, the mogroside (MG) bioconversion properties of several cell
wall structure defective mutants were analyzed. We also compared the
cell walls of these mutants by transmission electron microscopy, a
zymolyase sensitivity test, and a mannoprotein release assay. We found
zymolyase-sensitive mutants (including kre1Δ, las21Δ, gas1Δ, and kre6Δ), with defects in mannoprotein deposition, exhibit
efficient MG conversion and excessive leakage of Exg1; such defects
were not observed in wild-type cells, or mutants with abnormal levels
of glucans in the cell wall. Thus, yeast mutants defective in mannoprotein
deposition may be employed to convert glycosylated bioactive compounds.
%I ACS Publications