10.1021/acs.bioconjchem.5b00396.s001 Quanjuan Zhang Quanjuan Zhang Na Zhang Na Zhang Yi-Tao Long Yi-Tao Long Xuhong Qian Xuhong Qian Youjun Yang Youjun Yang Understanding the Selectivity of a Multichannel Fluorescent Probe for Peroxynitrite Over Hypochlorite American Chemical Society 2016 chemical community hypochlorite design probes peroxynitrite anion PN reactive nitrogen species nitric oxide cell injuries literature contributions OONO peroxynitrite probe 2016-02-10 15:47:21 Journal contribution https://acs.figshare.com/articles/journal_contribution/Understanding_the_Selectivity_of_a_Multichannel_Fluorescent_Probe_for_Peroxynitrite_Over_Hypochlorite/2077954 Peroxynitrite is a prominent biological reactive nitrogen species from radical combination of nitric oxide and superoxide and fundamentally involved in broad spectrum physiological and pathological processes. Though redox-inert itself, peroxynitrite anion (OONO<sup>–</sup>) attacks various biological electrophiles to generate an array of potent 2-e<sup>–</sup> or 1-e<sup>–</sup> oxidants, which result in cell injuries. Development of fluorescent probes for peroxynitrite, free from interference from hypochlorite, has been an active endeavor of the chemical community. We previously reported a peroxynitrite probe (<b>PN600</b>), which could differentiate hypochlorite from peroxynitrite through a multichannel signaling mechanism. Herein, this intriguing selectivity was accounted for through a structure–reactivity relationship study. Also, this work, together with rich literature contributions, has allowed a qualitative guideline in the use of electron-rich aromatic moieties to design probes against peroxynitrite and/or hypochlorite. The viability of this guideline was further testified by development of another list of peroxynitrite selective probes.