10.1021/ac5017776.s002
Ortal Schwartz
Ortal
Schwartz
Moran Bercovici
Moran
Bercovici
Microfluidic Assay for Continuous Bacteria Detection
Using Antimicrobial Peptides and Isotachophoresis
American Chemical Society
2015
technique
concentration AMPs reaction zone
Continuous Bacteria Detection
detection
leverage isotachophoresis
water samples
bacteria
antimicrobial peptides
concentration zone
fluorescence signal
microfluidic channel
concentration AMPs
novel microfluidic assay
water sample flows
Microfluidic Assay
Antimicrobial Peptides
1 h monitoring time
ITP
2015-12-17 05:20:07
Media
https://acs.figshare.com/articles/media/Microfluidic_Assay_for_Continuous_Bacteria_Detection_Using_Antimicrobial_Peptides_and_Isotachophoresis/2042397
We present a novel microfluidic assay
for continuous and quantitative
detection of bacteria in water. We leverage isotachophoresis (ITP),
an electrophoretic focusing technique, to create a stationary high
concentration zone of fluorescently labeled antimicrobial peptides
(AMPs) in a microfluidic channel. The tested water sample flows continuously
through this high concentration AMPs reaction zone; any bacteria present
in the sample is simultaneously labeled by, and separated from, the
high concentration AMPs. The labeled bacteria continue into the downstream
pure-buffer zone where the fluorescence signal is monitored, providing
a direct quantitative measurement of the original bacterial concentration
in the sample. We present the principles of the technique, demonstrate
its applicability for quantitative detection of <i>E. coli</i> as well as its stability over a 1 h monitoring time, and provide
a simple model for predicting its performance at different operating
conditions. The method could be potentially expanded for use with
other types of probes and provide continuous analysis and monitoring
of water samples at the point of need.