Mini 12, Miniature Mass Spectrometer for Clinical and Other ApplicationsIntroduction and Characterization Linfan Li Tsung-Chi Chen Yue Ren Paul I. Hendricks R. Graham Cooks Zheng Ouyang 10.1021/ac403766c.s001 https://acs.figshare.com/articles/journal_contribution/Mini_12_Miniature_Mass_Spectrometer_for_Clinical_and_Other_Applications_Introduction_and_Characterization/2030094 A benchtop miniature mass spectrometer system, Mini 12, with ambient ionization source and tandem mass spectrometry capabilities has been developed and characterized. This instrument was developed as a self-contained system to produce quantitative results for unprocessed samples of small volumes including nonvolatile analytes. The ion processing system, vacuum system, and control system are detailed. An integrated sample loading system facilitates automated operation. A user interface has been developed to acquire and to interpret analytical results for personnel who have limited mass spectrometry knowledge. Peak widths of Δ<i>m</i>/<i>z</i> 0.6 Th (full width at half-maximum) and a mass range of up to <i>m</i>/<i>z</i> 900 are demonstrated with the rectilinear ion trap mass analyzer. Multistage experiments up to MS<sup>5</sup> are accomplished. Consumable cartridges have been designed for use in ambient paper spray ionization, and the recently developed extraction spray ionization method has been employed to improve the quantitative performance. Monitoring of trace-levels of chemicals in therapeutic drugs, as well as in food safety and environmental protection operations is demonstrated. Dual MS/MS scans are implemented to obtain the intensities of the fragment ions from the analyte and its internal standard, and the ratio is used in quantitative analysis of complex samples. Limits of quantitation (LOQ) of 7.5 ng/mL, with relative standard deviations below 10%, have been obtained for selected therapeutic drugs in whole blood throughout their individual therapeutic ranges. 2015-12-17 01:03:46 Multistage experiments vacuum system user interface ion processing system unprocessed samples mass spectrometer system ion trap mass analyzer sample loading system MS 5 mass spectrometry knowledge food safety CharacterizationA benchtop control system nonvolatile analytes mass range ambient ionization source tandem mass spectrometry capabilities Consumable cartridges extraction spray ionization method peak widths LOQ ambient paper spray ionization protection operations fragment ions