Mini 12, Miniature Mass Spectrometer for Clinical
and Other Applicationsî—¸Introduction and Characterization
Linfan Li
Tsung-Chi Chen
Yue Ren
Paul I. Hendricks
R. Graham Cooks
Zheng Ouyang
10.1021/ac403766c.s001
https://acs.figshare.com/articles/journal_contribution/Mini_12_Miniature_Mass_Spectrometer_for_Clinical_and_Other_Applications_Introduction_and_Characterization/2030094
A benchtop miniature mass spectrometer
system, Mini 12, with ambient
ionization source and tandem mass spectrometry capabilities has been
developed and characterized. This instrument was developed as a self-contained
system to produce quantitative results for unprocessed samples of
small volumes including nonvolatile analytes. The ion processing system,
vacuum system, and control system are detailed. An integrated sample
loading system facilitates automated operation. A user interface has
been developed to acquire and to interpret analytical results for
personnel who have limited mass spectrometry knowledge. Peak widths
of Δ<i>m</i>/<i>z</i> 0.6 Th (full width
at half-maximum) and a mass range of up to <i>m</i>/<i>z</i> 900 are demonstrated with the rectilinear ion trap mass
analyzer. Multistage experiments up to MS<sup>5</sup> are accomplished.
Consumable cartridges have been designed for use in ambient paper
spray ionization, and the recently developed extraction spray ionization
method has been employed to improve the quantitative performance.
Monitoring of trace-levels of chemicals in therapeutic drugs, as well
as in food safety and environmental protection operations is demonstrated.
Dual MS/MS scans are implemented to obtain the intensities of the
fragment ions from the analyte and its internal standard, and the
ratio is used in quantitative analysis of complex samples. Limits
of quantitation (LOQ) of 7.5 ng/mL, with relative standard deviations
below 10%, have been obtained for selected therapeutic drugs in whole
blood throughout their individual therapeutic ranges.
2015-12-17 01:03:46
Multistage experiments
vacuum system
user interface
ion processing system
unprocessed samples
mass spectrometer system
ion trap mass analyzer
sample loading system
MS 5
mass spectrometry knowledge
food safety
CharacterizationA benchtop
control system
nonvolatile analytes
mass range
ambient ionization source
tandem mass spectrometry capabilities
Consumable cartridges
extraction spray ionization method
peak widths
LOQ
ambient paper spray ionization
protection operations
fragment ions