Lv, Haitao Hung, Chia S. Henderson, Jeffrey P. Metabolomic Analysis of Siderophore Cheater Mutants Reveals Metabolic Costs of Expression in Uropathogenic <i>Escherichia coli</i> Bacterial siderophores are a group of chemically diverse, virulence-associated secondary metabolites whose expression exerts metabolic costs. A combined bacterial genetic and metabolomic approach revealed differential metabolomic impacts associated with biosynthesis of different siderophore structural families. Despite myriad genetic differences, the metabolome of a cheater mutant lacking a single set of siderophore biosynthetic genes more closely approximate that of a non-pathogenic K12 strain than its isogenic, uropathogen parent strain. Siderophore types associated with greater metabolomic perturbations are less common among human isolates, suggesting that metabolic costs influence success in a human population. Although different siderophores share a common iron acquisition function, our analysis shows how a metabolomic approach can distinguish their relative metabolic impacts in <i>E. coli</i>. metabolomic approach;costs influence success;Siderophore Cheater Mutants;impact;Uropathogenic Escherichia coliBacterial siderophores;iron acquisition function;siderophore biosynthetic genes;uropathogen parent strain;K 12 strain 2015-12-17
    https://acs.figshare.com/articles/journal_contribution/Metabolomic_Analysis_of_Siderophore_Cheater_Mutants_Reveals_Metabolic_Costs_of_Expression_in_Uropathogenic_i_Escherichia_coli_i_/2029776
10.1021/pr4009749.s001