Complexin‑1
Enhances the On-Rate of Vesicle
Docking via Simultaneous SNARE and Membrane Interactions
Jiajie Diao
Daniel
J. Cipriano
Minglei Zhao
Yunxiang Zhang
Sachi Shah
Mark S. Padolina
Richard
A. Pfuetzner
Axel T. Brunger
10.1021/ja407392n.s001
https://acs.figshare.com/articles/journal_contribution/Complexin_1_Enhances_the_On_Rate_of_Vesicle_Docking_via_Simultaneous_SNARE_and_Membrane_Interactions/2026095
In
synaptic terminals, complexin is thought to have inhibitory
and activating roles for spontaneous “mini” release
and evoked synchronized neurotransmitter release, respectively. We
used single vesicle–vesicle microscopy imaging to study the
effect of complexin-1 on the on-rate of docking between vesicles that
mimic synaptic vesicles and the plasma membrane. We found that complexin-1
enhances the on-rate of docking of synaptic vesicle mimics containing
full-length synaptobrevin-2 and full-length synaptotagmin-1 to plasma
membrane-mimicking vesicles containing full-length syntaxin-1A and
SNAP-25A. This effect requires the C-terminal domain of complexin-1,
which binds to the membrane, the presence of PS in the membrane, and
the core region of complexin-1, which binds to the SNARE complex.
2015-12-16 23:41:06
core region
neurotransmitter release
plasma membrane
PS
docking
SNAP
synaptic vesicle mimics
complexin
Simultaneous SNARE
Membrane InteractionsIn synaptic terminals
synaptic vesicles
Vesicle Docking