10.1021/ja407392n.s001 Jiajie Diao Jiajie Diao Daniel J. Cipriano Daniel J. Cipriano Minglei Zhao Minglei Zhao Yunxiang Zhang Yunxiang Zhang Sachi Shah Sachi Shah Mark S. Padolina Mark S. Padolina Richard A. Pfuetzner Richard A. Pfuetzner Axel T. Brunger Axel T. Brunger Complexin‑1 Enhances the On-Rate of Vesicle Docking via Simultaneous SNARE and Membrane Interactions American Chemical Society 2015 core region neurotransmitter release plasma membrane PS docking SNAP synaptic vesicle mimics complexin Simultaneous SNARE Membrane InteractionsIn synaptic terminals synaptic vesicles Vesicle Docking 2015-12-16 23:41:06 Journal contribution https://acs.figshare.com/articles/journal_contribution/Complexin_1_Enhances_the_On_Rate_of_Vesicle_Docking_via_Simultaneous_SNARE_and_Membrane_Interactions/2026095 In synaptic terminals, complexin is thought to have inhibitory and activating roles for spontaneous “mini” release and evoked synchronized neurotransmitter release, respectively. We used single vesicle–vesicle microscopy imaging to study the effect of complexin-1 on the on-rate of docking between vesicles that mimic synaptic vesicles and the plasma membrane. We found that complexin-1 enhances the on-rate of docking of synaptic vesicle mimics containing full-length synaptobrevin-2 and full-length synaptotagmin-1 to plasma membrane-mimicking vesicles containing full-length syntaxin-1A and SNAP-25A. This effect requires the C-terminal domain of complexin-1, which binds to the membrane, the presence of PS in the membrane, and the core region of complexin-1, which binds to the SNARE complex.