The Membrane Protein LeuT in Micellar Systems: Aggregation
Dynamics and Detergent Binding to the S2 Site
George Khelashvili
Michael
V. LeVine
Lei Shi
Matthias Quick
Jonathan
A. Javitch
Harel Weinstein
10.1021/ja405984v.s001
https://acs.figshare.com/articles/journal_contribution/The_Membrane_Protein_LeuT_in_Micellar_Systems_Aggregation_Dynamics_and_Detergent_Binding_to_the_S2_Site/2025831
Structural
and functional properties of integral membrane proteins
are often studied in detergent micellar environments (proteomicelles),
but how such proteomicelles form and organize is not well understood.
This makes it difficult to evaluate the relationship between the properties
of the proteins measured in such a detergent-solubilized form and
under native conditions. To obtain mechanistic information about this
relationship for the leucine transporter (LeuT), a prokaryotic homologue
of the mammalian neurotransmitter/sodium symporters (NSSs), we studied
the properties of proteomicelles formed by <i>n</i>-dodecyl-β,d-maltopyranoside (DDM) detergent. Extensive atomistic molecular
dynamics simulations of different protein/detergent/water number ratios
revealed the formation of a proteomicelle characterized by a constant-sized
shell of detergents surrounding LeuT protecting its transmembrane
segments from unfavorable hydrophobic/hydrophilic exposure. Regardless
of the DDM content in the simulated system, this shell consisted of
a constant number of DDM molecules (∼120 measured at a 4 Å
cutoff distance from LeuT). In contrast, the overall number of DDMs
in the proteomicelle (aggregation number) was found to depend on the
detergent concentration, reaching a saturation value of 226±17
DDMs in the highest concentration regime simulated. Remarkably, we
found that at high detergent-to-protein ratios we observed two independent
ways of DDM penetration into LeuT, both leading to a positioning of
the DDM molecule in the second substrate (S2) binding site of LeuT.
Consonant with several recent experimental studies demonstrating changes
in functional properties of membrane proteins due to detergent, our
findings highlight how the environment in which the membrane proteins
are examined may affect the outcome and interpretation of their mechanistic
features.
2015-12-16 23:35:26
S 2 SiteStructural
detergent micellar environments
Membrane Protein LeuT
NSS
proteomicelle
membrane proteins
integral membrane proteins
4 Å cutoff distance
DDM