Gaster, Richard S. Hall, Drew A. Wang, Shan X. Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity We report an autoassembly protein array capable of rapidly screening for aberrant antibody−antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner. sensitivity;binding;manner;Autoassembly Protein Arrays;technique;identification;screening;nanosensor;method;15 min;autoassembly protein array;assay;nanoparticle technology;Antibody;50 fM;Analyzing;antibody 2015-12-16
    https://acs.figshare.com/articles/journal_contribution/Autoassembly_Protein_Arrays_for_Analyzing_Antibody_Cross_Reactivity/2016309
10.1021/nl1026056.s001